UCSC In-Silico PCR
 
>chr5:177408907-177409224 318bp TTTGGGTCTTGGGAAGAAATC GCTCACGATCACTCTAGTGCC
TTTGGGTCTTGGGAAGAAATCtctgcatttctgggctgtgagaggaagct
gcagactagcaacagatcggtggcaggctatgacttatagtcagttccct
gccttcttctctcccttgtagattccaccttgggaagcccccaaggagca
taagtacaaagctgaagagcacacagtcggtaagtggcctggctcctcct
cccgggaacccttgggtggggatgtgtatggtgcagtgtgtgcagtctca
gggcagtctagtctagtgcctacctggtgctaggtcttatgcccatgGGC
ACTAGAGTGATCGTGAGC

Primer Melting Temperatures
  Forward: 60.3 C tttgggtcttgggaagaaatc
Reverse: 60.0 C gctcacgatcactctagtgcc
The temperature calculations are done assuming 50 mM salt and 50 nM annealing oligo concentration. The code to calculate the melting temp comes from Primer3.

Help
  What is chr_alt & chr_fix?
Replicating in-Silico PCR results on local machine