UCSC In-Silico PCR

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UCSC In-Silico PCR

>chrIII:10813933+10814364 432bp ACCATTTCAGATGCAGACTTGGCGA CATCACATTTGTCGGAGTCGTCGGA ACCATTTCAGATGCAGACTTGGCGAcagcagttcctgatcccatagaaat tccgatttcggccttcttcaaagctggagcgtcgttgactccgtctccgg tcatagcagtaatctctccctgggattgaaggatatcgacaatcttggac ttgtgagatggctcgacacgggcgaaaagcttagctctgcggcaggcttc agattgttgctctggtggaagatcgtcaaattcacgtccagtgtaagctt ttccagtggtatcctcgttctctccgaagagtccgattcttcttccgata gcctcagcggtgttcttgttgtctccggtgatcatgatgacacggattcc agcgtggttacaagccttgatcgagtccgaaacttcagttcttggggggt caagcatTCCGACGACTCCGACAAATGTGATG

Primer Melting Temperatures


	`Forward: 69.9 C accatttcagatgcagacttggcga Reverse: 71.2 C catcacatttgtcggagtcgtcgga`The temperature calculations are done assuming 50 mM salt and 50 nM annealing oligo concentration. The code to calculate the melting temp comes from Primer3, the formula by Rychlik W, Spencer WJ and Rhoads RE NAR 1990, which can be activated in Primer3 with PRIMER_TM_FORMULA=0.

Help


	What is chr_alt & chr_fix? Replicating in-Silico PCR results on local machine