UCSC In-Silico PCR

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UCSC In-Silico PCR

>chrV:6530613+6531093 481bp TCAACACGTGATGATCCACCAGAGC TTTCCTTCAGCGCTTTCGTGCAATC TCAACACGTGATGATCCACCAGAGCaaaggatcaaatcagtcggacatat tatggatcacttggaagctgcaattgtagacaagagaaactgcattgttc cacgtggcgtaaaaggagaagttattgtccgtggatactcagttatgcgg tgctattggaacagtgaagagcagacaaagaaggaaatcactcaagacag atggtatcatactggagatattgctgttatgcatgataatggaaccatct ctattgttggaagatcgaaagatatgattgttcgtggaggagagaatatt tatcctaccgaagtagaacaatttttattcaagcatcagtcagtagaaga tgttcatgtaagcttcaggaaaatttcaaatttcaaaataaaattgaatt tcagattgtcggagtaccagatgaacgattcggtgaggtggtctgcgcct gggtcaGATTGCACGAAAGCGCTGAAGGAAA

Primer Melting Temperatures


	`Forward: 69.7 C tcaacacgtgatgatccaccagagc Reverse: 71.0 C tttccttcagcgctttcgtgcaatc`The temperature calculations are done assuming 50 mM salt and 50 nM annealing oligo concentration. The code to calculate the melting temp comes from Primer3, the formula by Rychlik W, Spencer WJ and Rhoads RE NAR 1990, which can be activated in Primer3 with PRIMER_TM_FORMULA=0.

Help


	What is chr_alt & chr_fix? Replicating in-Silico PCR results on local machine