This track shows gene trap sequence tags from GenBank
that have an RNA-based sequence tag method (5'-RACE or 3'-RACE) or
DNA-based sequence tag method. DNA-based gene trap sequence tags
(purple) are derived from genomic DNA at the site of gene trap vector
insertion. RNA-based gene trap sequence tags are derived from the
exon adjacent to the insertion site. If the sequence tag method is
5'-RACE (blue), the insertion site is in the intron downstream from
the most 3' exon represented in the sequence tag. If the sequence tag
is 3'-RACE (green), the insertion site is in the intron upstream from
the most 5' exon represented in the sequence tag.
Gene trap mutagenesis requires that the vector inserts in the correct
transcriptional orientation of the "trapped" gene. Sequence
tags that overlap known genes in the opposite transcriptional
orientation do not disrupt those genes from a gene trapping mechanism.
Sequence tags were collected from dbGSS and aligned to the reference
Informatics (MGI) at The Jackson Laboratory.
at The Jackson Laboratory,
and Bob Sinclair in particular, for providing these data.