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Micrococcal nuclease control coverage   (All Mapping and Sequencing tracks)

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 Cntl MNase +Covg  Micrococcal nuclease control coverage, sense strand reads   Schema 
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 Cntl MNase -Covg  Micrococcal nuclease control coverage, antisense strand reads   Schema 
Data coordinates converted via liftOver from: Jan. 2007 (WS170/ce4)

Description

Micrococcal nuclease control coverage, sense strand reads

The plot represents local coverage by control micrococcal nuclease fragments from cleavage of naked C. elegans DNA. By analogy with the experimental nucleosome data, 147 base pair sense segments starting from each of the individual control reads has been used to assemble a control coverage plot that accounts for enzymatic and sequencing biases of sampling from the reference genome.

Micrococcal nuclease control coverage, antisense strand reads

The plot represents local coverage by control micrococcal nuclease fragments from cleavage of naked C. elegans DNA. By analogy with the experimental nucleosome data, 147 base pair antisense segments starting from each of the individual control reads has been used to assemble a control coverage plot that accounts for enzymatic and sequencing biases of sampling from the reference genome.

Credits

The data for this track is supplied by the Sidow Lab and the Fire Lab at the Stanford School of Medicine.
Track display advice provided by Hiram Clawson, UCSC Genome Browser Engineering.

References

Valouev A, Ichikawa J, Tonthat T, Stuart J, Ranade S, Peckham H, Zeng K, Malek JA, Costa G, McKernan K et al. A high-resolution, nucleosome position map of C. elegans reveals a lack of universal sequence-dictated positioning. Genome Res. 2008 Jul;18(7):1051-63. PMID: 18477713; PMC: PMC2493394

Supported oligo ligation detection (SOLiD) sequencing technology from Applied Biosystems.