This track displays absolute transcript copy numbers for 136 genes and
12 negative control intergenic regions, determined by RTPCR in HCT116 cells.
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The genomic regions are indicated by solid blocks. The shade of an item gives a
rough indication of its count, ranging from light gray for zero to black for a
count of 7000 or greater. To display only those items that exceed a specific
unnormalized score, enter a minimum score between 0 and 1000 in the text box at
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Total RNA was prepared in quadruplicate from HCT116 cells grown in
culture. cDNA was prepared as described in Trinklein et
al. (2004). Duplicate primer pairs were designed to each gene, and the
absolute number of cDNA molecules containing each amplicon were
determined by real-time PCR. The submitted data are the calculated
number of molecules of each transcript containing the defined
Four biological replicates were performed, and two primer pairs were
used to measure the abundance of each transcript.
These data were generated in the Richard M. Myers lab
at Stanford University (now at
HudsonAlpha Institute for Biotechnology).
Trinklein, N.D., Chen, W.C., Kingston, R.E. and Myers, R.M.
Transcriptional regulation and binding of HSF1 and HSF2
to 32 human heat shock genes during thermal stress and
Cell Stress Chaperones 9(1), 21-28 (2004).