Description
This track shows gene expression differences between adult male
and female tissues, as described in Rinn et al., 2004.
Display Conventions and Configuration
In full display mode, the medians of all replicates (technical and biological)
for each sex's tissue are shown. To view the individual replicates, use the
UCSC Gene Sorter. In packed and squished
display modes, the average over all tissues is shown for each sex. Dense
display mode shows the placement of the Affy MOE430A target sequences
colored by overall expression level in both sexes, with darker colors
representing higher levels of expression.
Methods
Five adult mouse tissues (liver, kidney, hypothalamus, ovary and testis)
were studied. For each somatic tissue, triple selected poly-A mRNA was
prepared from six independent pools (biological replicates), three male and
three female. Likewise, three pools were prepared from the ovary and three
pools from the testis. Each pool of RNA was derived from ten individuals.
For each biological replicate, two cDNAs (technical replicates)
were prepared and independently hybridized to Affymetrix MOE430A chips.
Credits
Thanks to John Rinn for providing these data.
References
Rinn JL, Rozowsky JS, Laurenzi IJ, Petersen PH, Zou K, Zhong W, Gerstein M, Snyder M.
Major molecular differences between mammalian sexes are involved in drug metabolism and renal
function.
Dev Cell. 2004 Jun;6(6):791-800.
PMID: 15177028
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