Rinn Sex Exp Track Settings
 
Rinn et al. Sex Gene Expression Data on MOE430A Chip   (All Expression and Regulation tracks)

Display mode:      Duplicate track
Combine arrays:
Color:
red/green
red/blue
yellow/blue
red/blue on white background
red/blue on yellow background
Data schema/format description and download
Assembly: Mouse Feb. 2006 (NCBI36/mm8)
Data last updated at UCSC: 2006-03-09

Description

This track shows gene expression differences between adult male and female tissues, as described in Rinn et al., 2004.

Display Conventions and Configuration

In full display mode, the medians of all replicates (technical and biological) for each sex's tissue are shown. To view the individual replicates, use the UCSC Gene Sorter. In packed and squished display modes, the average over all tissues is shown for each sex. Dense display mode shows the placement of the Affy MOE430A target sequences colored by overall expression level in both sexes, with darker colors representing higher levels of expression.

Methods

Five adult mouse tissues (liver, kidney, hypothalamus, ovary and testis) were studied. For each somatic tissue, triple selected poly-A mRNA was prepared from six independent pools (biological replicates), three male and three female. Likewise, three pools were prepared from the ovary and three pools from the testis. Each pool of RNA was derived from ten individuals. For each biological replicate, two cDNAs (technical replicates) were prepared and independently hybridized to Affymetrix MOE430A chips.

Credits

Thanks to John Rinn for providing these data.

References

Rinn JL, Rozowsky JS, Laurenzi IJ, Petersen PH, Zou K, Zhong W, Gerstein M, Snyder M. Major molecular differences between mammalian sexes are involved in drug metabolism and renal function. Dev Cell. 2004 Jun;6(6):791-800. PMID: 15177028