GTEx Transcript Track Settings
 
Transcript Expression in 53 tissues from GTEx RNA-seq of 8555 samples/570 donors   (All Expression tracks)

Display mode:   

Label: Hugo gene name    Transcript identifier   

Log10(x+1) transform:    View limits maximum: TPM (range 0-8000)

Tissue types:  
 Adipose-Subcutaneous
 Adipose-Visceral_(Omentum)
 Adrenal_Gland
 Artery-Aorta
 Artery-Coronary
 Artery-Tibial
 Bladder
 Brain-Amygdala
 Brain-Anterior_cingulate_cortex_(BA24)
 Brain-Caudate_(basal_ganglia)
 Brain-Cerebellar_Hemisphere
 Brain-Cerebellum
 Brain-Cortex
 Brain-Frontal_Cortex_(BA9)
 Brain-Hippocampus
 Brain-Hypothalamus
 Brain-Nucleus_accumbens_(basal_ganglia)
 Brain-Putamen_(basal_ganglia)
 Brain-Spinal_cord_(cervical_c-1)
 Brain-Substantia_nigra
 Breast-Mammary_Tissue
 Cells-EBV-transformed_lymphocytes
 Cells-Transformed_fibroblasts
 Cervix-Ectocervix
 Cervix-Endocervix
 Colon-Sigmoid
 Colon-Transverse
 Esophagus-Gastroesophageal_Junction
 Esophagus-Mucosa
 Esophagus-Muscularis
 Fallopian_Tube
 Heart-Atrial_Appendage
 Heart-Left_Ventricle
 Kidney-Cortex
 Liver
 Lung
 Minor_Salivary_Gland
 Muscle-Skeletal
 Nerve-Tibial
 Ovary
 Pancreas
 Pituitary
 Prostate
 Skin-Not_Sun_Exposed_(Suprapubic)
 Skin-Sun_Exposed_(Lower_leg)
 Small_Intestine-Terminal_Ileum
 Spleen
 Stomach
 Testis
 Thyroid
 Uterus
 Vagina
 Whole_Blood
View table schema
Data last updated: 2017-05-23 12:10:30

Description

The NIH Genotype-Tissue Expression (GTEx) project was created to establish a sample and data resource for studies on the relationship between genetic variation and gene expression in multiple human tissues. This track displays median transcript expression levels in 53 tissues, based on RNA-seq data from the GTEx midpoint milestone data release (V6, October 2015). To view the GTEx tissues in anatomical context, see the GTEx Body Map.

Data for this track were computed at UCSC from GTEx RNA-seq sequence data using the Toil pipeline running the kallisto transcript-level quantification tool.

Display Conventions

In Full and Pack display modes, expression for each transcript is represented by a colored bar chart, where the height of each bar represents the median expression level across all samples for a tissue, and the bar color indicates the tissue.

The bar chart display has the same width and tissue order for all transcripts. Mouse hover over a bar will show the tissue and median expression level. The Squish display mode draws a rectangle for each gene, colored to indicate the tissue with highest expression level if it contributes more than 10% to the overall expression (and colored black if no tissue predominates). In Dense mode, the darkness of the grayscale rectangle displayed for the transcript reflects the total median expression level across all tissues.

Click-through on a graph displays a boxplot of expression level quartiles with outliers, per tissue.

Methods

Tissue samples were obtained using the GTEx standard operating procedures for informed consent and tissue collection, in conjunction with the National Cancer Institute Biorepositories and Biospecimen. All tissue specimens were reviewed by pathologists to characterize and verify organ source. Images from stained tissue samples can be viewed via the NCI histopathology viewer. The Qiagen PAXgene non-formalin tissue preservation product was used to stabilize tissue specimens without cross-linking biomolecules.

RNA-seq was performed by the GTEx Laboratory, Data Analysis and Coordinating Center (LDACC) at the Broad Institute. The Illumina TruSeq protocol was used to create an unstranded polyA+ library sequenced on the Illumina HiSeq 2000 platform to produce 76-bp paired end reads at a depth averaging 50M aligned reads per sample.

Sequence reads for this track were quantified to the hg38/GRCh38 human genome using kallisto assisted by the GENCODE v23 transcriptome definition. Read quantification was performed at UCSC by the Computational Genomics lab, using the Toil pipeline. The resulting kallisto files were combined to generate a transcript per million (TPM) expression matrix using the UCSC tool, kallistoToMatrix. Average TPM expression values for each tissue were calculated and used to generate a bed6+5 file that is the base of the track. This was done using the UCSC tool, expMatrixToBarchartBed. The bed track was then converted to a bigBed file using the UCSC tool, bedToBigBed.

The data in the hg19/GRCh37 version of this track was generated by converting the coordinates from the hg38/GRCh38 track data. Of the 189,615 BED entries from the original hg38 track, 176,220 were mapped over by transcript name to hg19 using wgEncodeGencodeCompV24lift37 (~93% coverage).

Subject and Sample Characteristics

The scientific goal of the GTEx project required that the donors and their biospecimen present with no evidence of disease. The tissue types collected were chosen based on their clinical significance, logistical feasibility and their relevance to the scientific goal of the project and the research community. Postmortem samples were collected from non-diseased donors with ages ranging from 20 to 79. 34.4% of donors were female and 65.6% male.

Additional summary plots of GTEx sample characteristics are available at the GTEx Portal Tissue Summary page.

Credits

Samples were collected by the GTEx Consortium. RNA-seq was performed by the GTEx Laboratory, Data Analysis and Coordinating Center (LDACC) at the Broad Institute. John Vivian, Melissa Cline, and Benedict Paten of the UCSC Computational Genomics lab were responsible for the sequence read quantification used to produce this track. Kate Rosenbloom and Chris Eisenhart of the UCSC Genome Browser group were responsible for data file post-processing and track configuration.

References

J. Vivian et al., Rapid and efficient analysis of 20,000 RNA-seq samples with Toil bioRxiv bioRxiv, vol. 2, p. 62497, 2016.

GTEx Consortium. The Genotype-Tissue Expression (GTEx) project. Nat Genet. 2013 Jun;45(6):580-5. PMID: 23715323; PMC: PMC4010069

Carithers LJ, Ardlie K, Barcus M, Branton PA, Britton A, Buia SA, Compton CC, DeLuca DS, Peter-Demchok J, Gelfand ET et al. A Novel Approach to High-Quality Postmortem Tissue Procurement: The GTEx Project. Biopreserv Biobank. 2015 Oct;13(5):311-9. PMID: 26484571; PMC: PMC4675181

Melé M, Ferreira PG, Reverter F, DeLuca DS, Monlong J, Sammeth M, Young TR, Goldmann JM, Pervouchine DD, Sullivan TJ et al. Human genomics. The human transcriptome across tissues and individuals. Science. 2015 May 8;348(6235):660-5. PMID: 25954002; PMC: PMC4547472

DeLuca DS, Levin JZ, Sivachenko A, Fennell T, Nazaire MD, Williams C, Reich M, Winckler W, Getz G. RNA-SeQC: RNA-seq metrics for quality control and process optimization. Bioinformatics. 2012 Jun 1;28(11):1530-2. PMID: 22539670; PMC: PMC3356847