Human Gene KRT33A (uc002hwk.2)
  Description: Homo sapiens keratin 33A (KRT33A), mRNA.
RefSeq Summary (NM_004138): This gene encodes a member of the keratin gene family. This gene is one of multiple type I hair keratin genes that are clustered in a region of chromosome 17q12-q21 and have the same direction of transcription. As a type I hair keratin, the encoded protein is an acidic protein which heterodimerizes with type II keratins to form hair and nails. There are two isoforms of this protein, encoded by two separate genes, keratin 33A and keratin 33B. [provided by RefSeq, May 2012].
Transcript (Including UTRs)
   Position: hg19 chr17:39,502,345-39,507,064 Size: 4,720 Total Exon Count: 7 Strand: -
Coding Region
   Position: hg19 chr17:39,502,371-39,507,019 Size: 4,649 Coding Exon Count: 7 

Page IndexSequence and LinksUniProtKB CommentsPrimersCTDGene Alleles
RNA-Seq ExpressionMicroarray ExpressionRNA StructureProtein StructureOther SpeciesGO Annotations
mRNA DescriptionsPathwaysOther NamesModel InformationMethods
Data last updated at UCSC: 2013-06-14

-  Sequence and Links to Tools and Databases
 
Genomic Sequence (chr17:39,502,345-39,507,064)mRNA (may differ from genome)Protein (404 aa)
Gene SorterGenome BrowserOther Species FASTAGene interactionsTable SchemaAlphaFold
BioGPSEnsemblEntrez GeneExonPrimerGeneCardsGeneNetwork
HGNCHPRDLynxMGIneXtProtOMIM
PubMedReactomeUniProtKBWikipediaBioGrid CRISPR DB

-  Comments and Description Text from UniProtKB
  ID: KT33A_HUMAN
DESCRIPTION: RecName: Full=Keratin, type I cuticular Ha3-I; AltName: Full=Hair keratin, type I Ha3-I; AltName: Full=Keratin-33A; Short=K33A;
TISSUE SPECIFICITY: Expressed in the hair follicles.
MISCELLANEOUS: There are two types of hair/microfibrillar keratin, I (acidic) and II (neutral to basic).
SIMILARITY: Belongs to the intermediate filament family.

-  Primer design for this transcript
 

Primer3Plus can design qPCR Primers that straddle exon-exon-junctions, which amplify only cDNA, not genomic DNA.
Click here to load the transcript sequence and exon structure into Primer3Plus

Exonprimer can design one pair of Sanger sequencing primers around every exon, located in non-genic sequence.
Click here to open Exonprimer with this transcript

To design primers for a non-coding sequence, zoom to a region of interest and select from the drop-down menu: View > In External Tools > Primer3


-  Comparative Toxicogenomics Database (CTD)
  The following chemicals interact with this gene

+  Common Gene Haplotype Alleles
  Press "+" in the title bar above to open this section.

-  RNA-Seq Expression Data from GTEx (53 Tissues, 570 Donors)
  Highest median expression: 3.10 RPKM in Testis
Total median expression: 4.10 RPKM



View in GTEx track of Genome Browser    View at GTEx portal     View GTEx Body Map

+  Microarray Expression Data
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-  mRNA Secondary Structure of 3' and 5' UTRs
 
RegionFold EnergyBasesEnergy/Base
Display As
5' UTR -7.1045-0.158 Picture PostScript Text
3' UTR -3.0026-0.115 Picture PostScript Text

The RNAfold program from the Vienna RNA Package is used to perform the secondary structure predictions and folding calculations. The estimated folding energy is in kcal/mol. The more negative the energy, the more secondary structure the RNA is likely to have.

-  Protein Domain and Structure Information
  InterPro Domains: Graphical view of domain structure
IPR016044 - F
IPR001664 - IF
IPR018039 - Intermediate_filament_CS
IPR002957 - Keratin_I

Pfam Domains:
PF00038 - Intermediate filament protein

ModBase Predicted Comparative 3D Structure on O76009
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The pictures above may be empty if there is no ModBase structure for the protein. The ModBase structure frequently covers just a fragment of the protein. You may be asked to log onto ModBase the first time you click on the pictures. It is simplest after logging in to just click on the picture again to get to the specific info on that model.

-  Orthologous Genes in Other Species
  Orthologies between human, mouse, and rat are computed by taking the best BLASTP hit, and filtering out non-syntenic hits. For more distant species reciprocal-best BLASTP hits are used. Note that the absence of an ortholog in the table below may reflect incomplete annotations in the other species rather than a true absence of the orthologous gene.
MouseRatZebrafishD. melanogasterC. elegansS. cerevisiae
No orthologGenome BrowserNo orthologNo orthologNo orthologNo ortholog
Gene DetailsGene Details    
Gene SorterGene Sorter    
 RGD    
 Protein Sequence    
 Alignment    

-  Gene Ontology (GO) Annotations with Structured Vocabulary
  Molecular Function:
GO:0005198 structural molecule activity

Biological Process:
GO:0031424 keratinization
GO:0070268 cornification

Cellular Component:
GO:0005615 extracellular space
GO:0005829 cytosol
GO:0005882 intermediate filament


-  Descriptions from all associated GenBank mRNAs
  AJ633621 - Homo sapiens mRNA for keratin hHa3-I (KRTHA3A gene).
BC069135 - Homo sapiens keratin 33A, mRNA (cDNA clone MGC:95416 IMAGE:7216991), complete cds.
AK314088 - Homo sapiens cDNA, FLJ94763, highly similar to Homo sapiens keratin, hair, acidic, 3A (KRTHA3A), mRNA.
JD157670 - Sequence 138694 from Patent EP1572962.

-  Biochemical and Signaling Pathways
  Reactome (by CSHL, EBI, and GO)

Protein O76009 (Reactome details) participates in the following event(s):

R-HSA-6805546 Keratin type I binds keratin type II
R-HSA-6805573 Keratin type I/type II heterodimers form tetramers
R-HSA-6806613 Keratin tetramers bind to form unit length filaments
R-HSA-6805567 Keratinization
R-HSA-1266738 Developmental Biology

-  Other Names for This Gene
  Alternate Gene Symbols: B2RA87, HHA3-I, HKA3A, KRTHA3A, KT33A_HUMAN, NM_004138, NP_004129, O76009, Q6NTB9, Q6ZZB9, uc002hwk.1
UCSC ID: uc002hwk.2
RefSeq Accession: NM_004138
Protein: O76009 (aka KT33A_HUMAN)
CCDS: CCDS11388.1

-  Gene Model Information
 
category: coding nonsense-mediated-decay: no RNA accession: NM_004138.3
exon count: 7CDS single in 3' UTR: no RNA size: 1287
ORF size: 1215CDS single in intron: no Alignment % ID: 100.00
txCdsPredict score: 2630.00frame shift in genome: no % Coverage: 99.92
has start codon: yes stop codon in genome: no # of Alignments: 1
has end codon: yes retained intron: no # AT/AC introns 0
selenocysteine: no end bleed into intron: 0# strange splices: 0
Click here for a detailed description of the fields of the table above.

-  Methods, Credits, and Use Restrictions
  Click here for details on how this gene model was made and data restrictions if any.