Description: Homo sapiens RAD52 homolog (S. cerevisiae) (RAD52), mRNA. RefSeq Summary (NM_134424): The protein encoded by this gene shares similarity with Saccharomyces cerevisiae Rad52, a protein important for DNA double-strand break repair and homologous recombination. This gene product was shown to bind single-stranded DNA ends, and mediate the DNA-DNA interaction necessary for the annealing of complementary DNA strands. It was also found to interact with DNA recombination protein RAD51, which suggested its role in RAD51 related DNA recombination and repair. A pseudogene of this gene is present on chromosome 2. Alternative splicing results in multiple transcript variants. Additional alternatively spliced transcript variants of this gene have been described, but their full-length nature is not known. [provided by RefSeq, Jul 2014]. Transcript (Including UTRs) Position: hg19 chr12:1,021,255-1,058,863 Size: 37,609 Total Exon Count: 12 Strand: - Coding Region Position: hg19 chr12:1,022,557-1,042,224 Size: 19,668 Coding Exon Count: 11
ID:RAD52_HUMAN DESCRIPTION: RecName: Full=DNA repair protein RAD52 homolog; FUNCTION: Involved in double-stranded break repair. Plays a central role in genetic recombination and DNA repair by promoting the annealing of complementary single-stranded DNA and by stimulation of the RAD51 recombinase. SUBUNIT: Forms a undecameric ring. Interacts with ABL1. INTERACTION: Q06609:RAD51; NbExp=3; IntAct=EBI-706448, EBI-297202; P35244:RPA3; NbExp=2; IntAct=EBI-706448, EBI-621428; Q14191:WRN; NbExp=9; IntAct=EBI-706448, EBI-368417; SUBCELLULAR LOCATION: Nucleus (Potential). PTM: Phosphorylated upon DNA damage by ABL1, and probably by ATM or ATR. SIMILARITY: Belongs to the RAD52 family. WEB RESOURCE: Name=NIEHS-SNPs; URL="http://egp.gs.washington.edu/data/rad52/";
The RNAfold program from the Vienna RNA Package is used to perform the secondary structure predictions and folding calculations. The estimated folding energy is in kcal/mol. The more negative the energy, the more secondary structure the RNA is likely to have.
ModBase Predicted Comparative 3D Structure on P43351
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Orthologous Genes in Other Species
Orthologies between human, mouse, and rat are computed by taking the best BLASTP hit, and filtering out non-syntenic hits. For more distant species reciprocal-best BLASTP hits are used. Note that the absence of an ortholog in the table below may reflect incomplete annotations in the other species rather than a true absence of the orthologous gene.
Biological Process: GO:0000724 double-strand break repair via homologous recombination GO:0000730 DNA recombinase assembly GO:0006281 DNA repair GO:0006302 double-strand break repair GO:0006310 DNA recombination GO:0006974 cellular response to DNA damage stimulus GO:0010792 DNA double-strand break processing involved in repair via single-strand annealing GO:0034599 cellular response to oxidative stress GO:0045002 double-strand break repair via single-strand annealing GO:0051260 protein homooligomerization GO:2000819 regulation of nucleotide-excision repair
BC042136 - Homo sapiens RAD52 homolog (S. cerevisiae), mRNA (cDNA clone IMAGE:5802809), with apparent retained intron. AF125948 - Homo sapiens DNA repair protein RAD52 delta isoform (RAD52) mRNA, alternatively spliced, complete cds. L33262 - Homo sapiens DNA repair and recombination homologue (RAD52) gene, complete cds. AF125949 - Homo sapiens DNA repair protein RAD52 gamma isoform (RAD52) mRNA, alternatively spliced, complete cds. AK290047 - Homo sapiens cDNA FLJ77029 complete cds, highly similar to Homo sapiens RAD52 homolog (S. cerevisiae) (RAD52), transcript variant alpha, mRNA. AK299283 - Homo sapiens cDNA FLJ57177 complete cds, highly similar to DNA repair protein RAD52 homolog. BC114507 - Homo sapiens RAD52 homolog (S. cerevisiae), mRNA (cDNA clone IMAGE:40024511). BC104015 - Homo sapiens RAD52 homolog (S. cerevisiae), mRNA (cDNA clone IMAGE:40024504), partial cds. BC104016 - Homo sapiens RAD52 homolog (S. cerevisiae), mRNA (cDNA clone IMAGE:40024507). BC104017 - Homo sapiens RAD52 homolog (S. cerevisiae), mRNA (cDNA clone IMAGE:40024509), partial cds. BC114954 - Homo sapiens RAD52 homolog (S. cerevisiae), mRNA (cDNA clone IMAGE:40024512), complete cds. AK294709 - Homo sapiens cDNA FLJ56674 complete cds, highly similar to DNA repair protein RAD52 homolog. AK292160 - Homo sapiens cDNA FLJ76573 complete cds, highly similar to Homo sapiens RAD52 homolog (S. cerevisiae) (RAD52), transcript variant gamma, mRNA. AK312026 - Homo sapiens cDNA, FLJ92302, Homo sapiens RAD52 homolog (S. cerevisiae) (RAD52), transcriptvariant delta, mRNA. U12134 - Human DNA damage repair and recombination protein RAD52 mRNA, complete cds. U27516 - Human recombination protein RAD52 mRNA, complete cds. BC144458 - Homo sapiens cDNA clone IMAGE:9052984. JD172470 - Sequence 153494 from Patent EP1572962. JD539568 - Sequence 520592 from Patent EP1572962. JD523641 - Sequence 504665 from Patent EP1572962. JD042671 - Sequence 23695 from Patent EP1572962. JD215068 - Sequence 196092 from Patent EP1572962. JD083432 - Sequence 64456 from Patent EP1572962. JD047033 - Sequence 28057 from Patent EP1572962. JD427305 - Sequence 408329 from Patent EP1572962. JD241332 - Sequence 222356 from Patent EP1572962. JD356864 - Sequence 337888 from Patent EP1572962. JD313398 - Sequence 294422 from Patent EP1572962. JD121135 - Sequence 102159 from Patent EP1572962. JD456516 - Sequence 437540 from Patent EP1572962. JD262215 - Sequence 243239 from Patent EP1572962. JD376234 - Sequence 357258 from Patent EP1572962. JD515941 - Sequence 496965 from Patent EP1572962. JD293653 - Sequence 274677 from Patent EP1572962. JD467500 - Sequence 448524 from Patent EP1572962. JD192571 - Sequence 173595 from Patent EP1572962. JD550399 - Sequence 531423 from Patent EP1572962. JD329801 - Sequence 310825 from Patent EP1572962. JD441728 - Sequence 422752 from Patent EP1572962. AY540753 - Homo sapiens rhabdomyosarcoma antigen MU-RMS-40.23 (RAD52) mRNA, complete cds. JD363125 - Sequence 344149 from Patent EP1572962. JD046149 - Sequence 27173 from Patent EP1572962. JD372371 - Sequence 353395 from Patent EP1572962. JD288239 - Sequence 269263 from Patent EP1572962. JD557107 - Sequence 538131 from Patent EP1572962. JD454242 - Sequence 435266 from Patent EP1572962. JD052003 - Sequence 33027 from Patent EP1572962. JD267448 - Sequence 248472 from Patent EP1572962. JD510925 - Sequence 491949 from Patent EP1572962. BC160123 - Synthetic construct Homo sapiens clone IMAGE:100064155, MGC:193238 RAD52 homolog (S. cerevisiae) (RAD52) mRNA, encodes complete protein. AB528581 - Synthetic construct DNA, clone: pF1KB0498, Homo sapiens RAD52 gene for RAD52 homolog, without stop codon, in Flexi system. JD276870 - Sequence 257894 from Patent EP1572962. JD203932 - Sequence 184956 from Patent EP1572962. JD195461 - Sequence 176485 from Patent EP1572962. JD492586 - Sequence 473610 from Patent EP1572962. JD309660 - Sequence 290684 from Patent EP1572962. JD068975 - Sequence 49999 from Patent EP1572962. JD394808 - Sequence 375832 from Patent EP1572962. JD463687 - Sequence 444711 from Patent EP1572962. JD128975 - Sequence 109999 from Patent EP1572962. JD050970 - Sequence 31994 from Patent EP1572962. JD355565 - Sequence 336589 from Patent EP1572962. AF125950 - Homo sapiens DNA repair protein RAD52 beta isoform (RAD52) mRNA, alternatively spliced, complete cds. JD560068 - Sequence 541092 from Patent EP1572962. JD182899 - Sequence 163923 from Patent EP1572962. JD077853 - Sequence 58877 from Patent EP1572962. JD378908 - Sequence 359932 from Patent EP1572962. JD450438 - Sequence 431462 from Patent EP1572962. CQ873742 - Sequence 161 from Patent WO2004076622. DD413579 - Regulation of Mammalian Cells. JD251509 - Sequence 232533 from Patent EP1572962.
Biochemical and Signaling Pathways
KEGG - Kyoto Encyclopedia of Genes and Genomes hsa03440 - Homologous recombination
Reactome (by CSHL, EBI, and GO)
Protein P43351 (Reactome details) participates in the following event(s):
R-HSA-75998 Formation of RAD52 heptameric ring structure complexes R-HSA-4568863 SUMOylation of RAD52 with SUMO1 R-HSA-5686587 ABL1 phosphorylates RAD52 R-HSA-5693580 Association of RAD52 with the RPA complex at resected DNA DSBs R-HSA-5686657 ERCC1:XPF cleaves flaps generated by SSA R-HSA-5693564 Association of RAD51 with RAD52:DNA double-strand break ends R-HSA-5686642 RAD52 promotes single strand annealing at resected DNA DSBs R-HSA-5685938 HDR through Single Strand Annealing (SSA) R-HSA-3108214 SUMOylation of DNA damage response and repair proteins R-HSA-5693567 HDR through Homologous Recombination (HR) or Single Strand Annealing (SSA) R-HSA-3108232 SUMO E3 ligases SUMOylate target proteins R-HSA-5693538 Homology Directed Repair R-HSA-2990846 SUMOylation R-HSA-5693532 DNA Double-Strand Break Repair R-HSA-597592 Post-translational protein modification R-HSA-73894 DNA Repair R-HSA-392499 Metabolism of proteins