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Human Gene ALDOB (ENST00000647789.2) from GENCODE V50
  Description: aldolase, fructose-bisphosphate B (from RefSeq NM_000035.4)
Gencode Transcript: ENST00000647789.2
Gencode Gene: ENSG00000136872.23
Transcript (Including UTRs)
   Position: hg38 chr9:101,420,560-101,435,774 Size: 15,215 Total Exon Count: 9 Strand: -
Coding Region
   Position: hg38 chr9:101,421,809-101,430,887 Size: 9,079 Coding Exon Count: 8 

Page IndexSequence and LinksUniProtKB CommentsPrimersMalaCardsCTD
RNA-Seq ExpressionMicroarray ExpressionRNA StructureProtein StructureOther SpeciesGO Annotations
mRNA DescriptionsPathwaysOther NamesGeneReviewsMethods
Data last updated at UCSC: 2026-07-12 09:30:09

-  Comments and Description Text from UniProtKB
  ID: ALDOB_HUMAN
DESCRIPTION: RecName: Full=Fructose-bisphosphate aldolase B; EC=4.1.2.13; AltName: Full=Liver-type aldolase;
CATALYTIC ACTIVITY: D-fructose 1,6-bisphosphate = glycerone phosphate + D-glyceraldehyde 3-phosphate.
BIOPHYSICOCHEMICAL PROPERTIES: Kinetic parameters: KM=1.6 uM for fructose 1,6-bisphosphate; KM=2.3 mM for fructose 1-phosphate;
PATHWAY: Carbohydrate degradation; glycolysis; D-glyceraldehyde 3- phosphate and glycerone phosphate from D-glucose: step 4/4.
SUBUNIT: Homotetramer.
INTERACTION: Q8NFJ9:BBS1; NbExp=4; IntAct=EBI-1045507, EBI-1805484; Q9BXC9:BBS2; NbExp=4; IntAct=EBI-1045507, EBI-748297; Q96RK4:BBS4; NbExp=4; IntAct=EBI-1045507, EBI-1805814; Q8IWZ6:BBS7; NbExp=4; IntAct=EBI-1045507, EBI-1806001;
DISEASE: Defects in ALDOB are the cause of hereditary fructose intolerance (HFI) [MIM:229600]. HFI is an autosomal recessive disease that results in an inability to metabolize fructose and related sugars. Complete exclusion of fructose results in dramatic recovery; however, if not treated properly, HFI subjects suffer episodes of hypoglycemia, general ill condition, and risk of death the remainder of life.
MISCELLANEOUS: In vertebrates, three forms of this ubiquitous glycolytic enzyme are found, aldolase A in muscle, aldolase B in liver and aldolase C in brain.
SIMILARITY: Belongs to the class I fructose-bisphosphate aldolase family.
WEB RESOURCE: Name=Atlas of Genetics and Cytogenetics in Oncology and Haematology; URL="http://atlasgeneticsoncology.org/Genes/ALDOBID44287ch9q31.html";
WEB RESOURCE: Name=GeneReviews; URL="http://www.ncbi.nlm.nih.gov/sites/GeneTests/lab/gene/ALDOB";

-  Primer design for this transcript
 

Primer3Plus can design qPCR Primers that straddle exon-exon-junctions, which amplify only cDNA, not genomic DNA.
Click here to load the transcript sequence and exon structure into Primer3Plus

Exonprimer can design one pair of Sanger sequencing primers around every exon, located in non-genic sequence.
Click here to open Exonprimer with this transcript

To design primers for a non-coding sequence, zoom to a region of interest and select from the drop-down menu: View > In External Tools > Primer3


-  MalaCards Disease Associations
  MalaCards Gene Search: ALDOB
Diseases sorted by gene-association score: fructose intolerance, hereditary* (1697), posterior myocardial infarction (15), fructose-1,6-bisphosphatase deficiency (13), sedoheptulokinase deficiency (13), tropical sprue (11), lactose intolerance (8), small intestine lymphoma (7), galactosemia (6), glycogen storage disease ia (6), autosomal recessive disease (6), lymphocytic colitis (5), carbohydrate metabolic disorder (5), xanthinuria (4)
* = Manually curated disease association

-  Comparative Toxicogenomics Database (CTD)
  The following chemicals interact with this gene           more ... click here to view the complete list

-  RNA-Seq Expression Data from GTEx (53 Tissues, 570 Donors)
  Highest median expression: 1558.10 RPKM in Liver
Total median expression: 3307.30 RPKM



View in GTEx track of Genome Browser    View at GTEx portal     View GTEx Body Map

+  Microarray Expression Data
 
Expression ratio colors:

GNF Expression Atlas 2 Data from U133A and GNF1H Chips

      
      
      
     
    
     
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-  mRNA Secondary Structure of 3' and 5' UTRs
 
RegionFold EnergyBasesEnergy/Base
Display As
5' UTR -13.4076-0.176 Picture PostScript Text
3' UTR -270.301249-0.216 Picture PostScript Text

The RNAfold program from the Vienna RNA Package is used to perform the secondary structure predictions and folding calculations. The estimated folding energy is in kcal/mol. The more negative the energy, the more secondary structure the RNA is likely to have.

-  Protein Domain and Structure Information
  InterPro Domains: Graphical view of domain structure
IPR000741 - Aldolase_I
IPR013785 - Aldolase_TIM

Pfam Domains:
PF00274 - Fructose-bisphosphate aldolase class-I

Protein Data Bank (PDB) 3-D Structure
MuPIT help
1QO5 - X-ray MuPIT 1XDL - X-ray MuPIT 1XDM - X-ray MuPIT


ModBase Predicted Comparative 3D Structure on P05062
FrontTopSide
The pictures above may be empty if there is no ModBase structure for the protein. The ModBase structure frequently covers just a fragment of the protein. You may be asked to log onto ModBase the first time you click on the pictures. It is simplest after logging in to just click on the picture again to get to the specific info on that model.

-  Orthologous Genes in Other Species
  Orthologies between human, mouse, and rat are computed by taking the best BLASTP hit, and filtering out non-syntenic hits. For more distant species reciprocal-best BLASTP hits are used. Note that the absence of an ortholog in the table below may reflect incomplete annotations in the other species rather than a true absence of the orthologous gene.
MouseRatZebrafishD. melanogasterC. elegansS. cerevisiae
Genome BrowserGenome BrowserNo orthologNo orthologNo orthologNo ortholog
Gene Details     
Gene Sorter     
MGIRGD    
Protein SequenceProtein Sequence    
AlignmentAlignment    

-  Gene Ontology (GO) Annotations with Structured Vocabulary
  Molecular Function:
GO:0003824 catalytic activity
GO:0004332 fructose-bisphosphate aldolase activity
GO:0005515 protein binding
GO:0008092 cytoskeletal protein binding
GO:0016829 lyase activity
GO:0042802 identical protein binding
GO:0051117 ATPase binding
GO:0061609 fructose-1-phosphate aldolase activity
GO:0070061 fructose binding

Biological Process:
GO:0006000 fructose metabolic process
GO:0006094 gluconeogenesis
GO:0006096 glycolytic process
GO:0006116 NADH oxidation
GO:0030388 fructose 1,6-bisphosphate metabolic process
GO:0032781 positive regulation of ATPase activity
GO:0061621 canonical glycolysis
GO:0061624 fructose catabolic process to hydroxyacetone phosphate and glyceraldehyde-3-phosphate
GO:0070072 vacuolar proton-transporting V-type ATPase complex assembly

Cellular Component:
GO:0005737 cytoplasm
GO:0005815 microtubule organizing center
GO:0005829 cytosol
GO:0005856 cytoskeleton
GO:0034451 centriolar satellite
GO:0070062 extracellular exosome


-  Descriptions from all associated GenBank mRNAs
  BC005314 - Homo sapiens mRNA similar to haptoglobin (cDNA clone IMAGE:3934411).
X01098 - Human mRNA for aldolase B (EC 4.1.2.13).
AK026411 - Homo sapiens cDNA: FLJ22758 fis, clone KAIA0811, highly similar to HUMALDB Human aldolase B mRNA.
X02747 - Human mRNA for aldolase B.
AK290795 - Homo sapiens cDNA FLJ76146 complete cds, highly similar to Homo sapiens aldolase B, fructose-bisphosphate (ALDOB), mRNA.
JD376219 - Sequence 357243 from Patent EP1572962.
JD113140 - Sequence 94164 from Patent EP1572962.
JD349980 - Sequence 331004 from Patent EP1572962.
BC029399 - Homo sapiens aldolase B, fructose-bisphosphate, mRNA (cDNA clone MGC:32618 IMAGE:4593670), complete cds.
X00270 - Human human aldolase B messenger RNA fragment (EC 4.1.2.13).
KJ901281 - Synthetic construct Homo sapiens clone ccsbBroadEn_10675 ALDOB gene, encodes complete protein.
KR711264 - Synthetic construct Homo sapiens clone CCSBHm_00021956 ALDOB (ALDOB) mRNA, encodes complete protein.
KR711265 - Synthetic construct Homo sapiens clone CCSBHm_00021963 ALDOB (ALDOB) mRNA, encodes complete protein.
KR711266 - Synthetic construct Homo sapiens clone CCSBHm_00021970 ALDOB (ALDOB) mRNA, encodes complete protein.
KR711267 - Synthetic construct Homo sapiens clone CCSBHm_00021974 ALDOB (ALDOB) mRNA, encodes complete protein.
JD349991 - Sequence 331015 from Patent EP1572962.
JD187663 - Sequence 168687 from Patent EP1572962.

-  Biochemical and Signaling Pathways
  KEGG - Kyoto Encyclopedia of Genes and Genomes
hsa00010 - Glycolysis / Gluconeogenesis
hsa00030 - Pentose phosphate pathway
hsa00051 - Fructose and mannose metabolism
hsa01100 - Metabolic pathways

BioCyc Knowledge Library
PWY66-373 - sucrose degradation
PWY66-399 - gluconeogenesis
PWY66-400 - glycolysis
PWY66-407 - superpathway of conversion of glucose to acetyl CoA and entry into the TCA cycle

Reactome (by CSHL, EBI, and GO)

Protein P05062 (Reactome details) participates in the following event(s):

R-HSA-70342 ALDOB tetramer cleaves Fru-1-P to GA and DHAP
R-HSA-71495 dihydroxyacetone phosphate + D-glyceraldehyde 3-phosphate <=> D-fructose 1,6-bisphosphate
R-HSA-71496 D-fructose 1,6-bisphosphate <=> dihydroxyacetone phosphate + D-glyceraldehyde 3-phosphate
R-HSA-70350 Fructose catabolism
R-HSA-70263 Gluconeogenesis
R-HSA-70171 Glycolysis
R-HSA-5652084 Fructose metabolism
R-HSA-70326 Glucose metabolism
R-HSA-71387 Metabolism of carbohydrates
R-HSA-1430728 Metabolism

-  Other Names for This Gene
  Alternate Gene Symbols: ALDB, ALDOB , ALDOB_HUMAN, ENST00000647789.1, NM_000035, P05062, Q13741, Q13742, Q5T7D6, uc286ccw.1, uc286ccw.2
UCSC ID: ENST00000647789.2
RefSeq Accession: NM_000035.4
Protein: P05062 (aka ALDOB_HUMAN or ALFB_HUMAN)
CCDS: CCDS6756.1

-  GeneReviews for This Gene
  GeneReviews article(s) related to gene ALDOB:
hfi (Hereditary Fructose Intolerance)

-  Methods, Credits, and Use Restrictions
  Click here for details on how this gene model was made and data restrictions if any.