Human Gene BRAF (ENST00000644969.2) from GENCODE V50

Description: B-Raf proto-oncogene, serine/threonine kinase, transcript variant 5 (from RefSeq NM_001374258.1)
Gencode Transcript: ENST00000644969.2
Gencode Gene: ENSG00000157764.16
Transcript (Including UTRs)
Position: hg38 chr7:140,719,337-140,924,929 Size: 205,593 Total Exon Count: 20 Strand: -
Coding Region
Position: hg38 chr7:140,726,494-140,924,703 Size: 198,210 Coding Exon Count: 20

Page Index	Sequence and Links	Primers	MalaCards	CTD	RNA-Seq Expression
Microarray Expression	RNA Structure	Other Species	mRNA Descriptions	Pathways	Other Names
GeneReviews	Methods

Data last updated at UCSC: 2026-07-12 09:30:09

Sequence and Links to Tools and Databases

Genomic Sequence (chr7:140,719,337-140,924,929)			mRNA (may differ from genome)		Protein (807 aa)
Gene Sorter	Genome Browser	Other Species FASTA	Gene interactions	Table Schema	AlphaFold
BioGPS	Ensembl	Entrez Gene	ExonPrimer	Gencode	GeneCards
HGNC	Lynx	Malacards	MGI	myGene2	OMIM
PubMed	UniProtKB	Wikipedia	BioGrid CRISPR DB

Primer design for this transcript

Primer3Plus can design qPCR Primers that straddle exon-exon-junctions, which amplify only cDNA, not genomic DNA.
Click here to load the transcript sequence and exon structure into Primer3Plus

Exonprimer can design one pair of Sanger sequencing primers around every exon, located in non-genic sequence.
Click here to open Exonprimer with this transcript

To design primers for a non-coding sequence, zoom to a region of interest and select from the drop-down menu: View > In External Tools > Primer3

MalaCards Disease Associations


	MalaCards Gene Search: BRAF Diseases sorted by gene-association score: cardiofaciocutaneous syndrome* (1601), lung cancer* (1278), noonan syndrome 7* (1229), colorectal cancer* (992), leopard syndrome 3* (829), braf-related noonan syndrome* (500), noonan syndrome 1* (385), hashimoto-pritzker syndrome* (350), classic hairy cell leukemia* (350), melanoma* (323), craniopharyngioma* (253), cardiomyopathy, hypertrophic, 4* (231), ptpn11-related noonan syndrome* (231), pilocytic astrocytoma* (208), colonic benign neoplasm* (200), noonan syndrome with multiple lentigines* (175), lymphoma, non-hodgkin* (122), thyroid carcinoma somatic* (117), braf-related cardiofaciocutaneous syndrome* (100), braf-related leopard syndrome* (100), differentiated thyroid carcinoma* (88), adenocarcinoma (68), malignant melanoma, somatic* (45), thyroid cancer (35), spitz nevus (25), erdheim-chester disease (25), hodgkin lymphoma (25), sarcoma (24), hairy cell leukemia (22), thyroid cancer, nonmedullary, 1 (19), leopard syndrome (19), desmoplastic infantile astrocytoma/ganglioglioma (19), desmoplastic infantile astrocytoma (18), syringocystadenoma papilliferum (18), refractory hairy cell leukemia (18), myopericytoma (18), thyroid cancer, anaplastic (14), colorectal adenocarcinoma (14), pulmonic stenosis (13), papillary carcinoma (13), papillary craniopharyngioma (13), nevus of ota (13), granulomatous dermatitis (13), nephrotic syndrome, type 3 (13), metanephric adenoma (11), rhabdoid meningioma (10), pleomorphic xanthoastrocytoma (10), struma ovarii (9), lynch syndrome (9), adenofibroma (9), histiocytosis (8), adenoma (8), skin melanoma (8), hyperplastic polyposis syndrome (8), small intestinal adenocarcinoma (8), mature teratoma (8), pilocytic astrocytoma of cerebellum (8), mucosal melanoma (8), ganglioglioma (8), papillary tumor of the pineal region (7), juvenile myelomonocytic leukemia (7), lip cancer (7), amelanotic melanoma (7), cerebellar astrocytoma (7), uveal melanoma (6), villous adenoma (6), lymphoma (6), histiocytic sarcoma (6), nodular goiter (6), hereditary colorectal cancer (5), thyroid cancer, nonmedullary, 2 (5), letterer-siwe disease (5), familial colorectal cancer (5), giant cell glioblastoma (4), endometrial cancer (3), renal cell carcinoma (2), lung cancer susceptibility 3 (1) * = Manually curated disease association

Comparative Toxicogenomics Database (CTD)


	The following chemicals interact with this gene C471405 sorafenib D000082 Acetaminophen D000242 Cyclic AMP C551177 PLX4032 D010634 Phenobarbital C028474 1,4-bis(2-(3,5-dichloropyridyloxy))benzene C111118 2',3,3',4',5-pentachloro-4-hydroxybiphenyl C078765 2,3,5-(triglutathion-S-yl)hydroquinone C023514 2,6-dinitrotoluene C532162 2-(1H-indazol-4-yl)-6-(4-methanesulfonylpiperazin-1-ylmethyl)-4-morpholin-4-ylthieno(3,2-d)pyrimidine more ... click here to view the complete list

RNA-Seq Expression Data from GTEx (53 Tissues, 570 Donors)


	Highest median expression: 13.20 RPKM in Testis Total median expression: 148.18 RPKM View in GTEx track of Genome Browser View at GTEx portal View GTEx Body Map

Microarray Expression Data

mRNA Secondary Structure of 3' and 5' UTRs

Region	Fold Energy	Bases	Energy/Base	Display As
5' UTR	-82.10	226	-0.363	Picture	PostScript	Text
3' UTR	-1853.00	7157	-0.259	Picture	PostScript	Text

The RNAfold program from the Vienna RNA Package is used to perform the secondary structure predictions and folding calculations. The estimated folding energy is in kcal/mol. The more negative the energy, the more secondary structure the RNA is likely to have.

Orthologous Genes in Other Species

Orthologies between human, mouse, and rat are computed by taking the best BLASTP hit, and filtering out non-syntenic hits. For more distant species reciprocal-best BLASTP hits are used. Note that the absence of an ortholog in the table below may reflect incomplete annotations in the other species rather than a true absence of the orthologous gene.

Mouse	Rat	Zebrafish	D. melanogaster	C. elegans	S. cerevisiae
Genome Browser	Genome Browser	Genome Browser	No ortholog	Genome Browser	No ortholog
Gene Details
Gene Sorter
MGI	RGD	Ensembl		WormBase
Protein Sequence	Protein Sequence	Protein Sequence		Protein Sequence
Alignment	Alignment	Alignment		Alignment

Descriptions from all associated GenBank mRNAs


	HM053972 - Homo sapiens AGTRAP-BRAF fusion protein mRNA, complete cds. GU149303 - Homo sapiens SLC45A3-BRAF fusion protein mRNA, complete cds. BC038966 - Homo sapiens, Similar to v-raf murine sarcoma viral oncogene homolog B1, clone IMAGE:4391530, mRNA. M95712 - Homo sapiens B-raf protein (BRAF) mRNA, complete cds. BC101757 - Homo sapiens v-raf murine sarcoma viral oncogene homolog B1, mRNA (cDNA clone MGC:126806 IMAGE:8069263), complete cds. BC112079 - Homo sapiens v-raf murine sarcoma viral oncogene homolog B1, mRNA (cDNA clone MGC:138284 IMAGE:8327547), complete cds. M21001 - Human B-raf oncogene mRNA, 3' end. AK314346 - Homo sapiens cDNA, FLJ95109. KJ890780 - Synthetic construct Homo sapiens clone ccsbBroadEn_00174 BRAF gene, encodes complete protein. KR712180 - Synthetic construct Homo sapiens clone CCSBHm_00036663 BRAF (BRAF) mRNA, encodes complete protein. AB529216 - Synthetic construct DNA, clone: pF1KB5776, Homo sapiens BRAF gene for v-raf murine sarcoma viral oncogene homolog B1, without stop codon, in Flexi system. AK094023 - Homo sapiens cDNA FLJ36704 fis, clone UTERU2009335. AK098095 - Homo sapiens cDNA FLJ40776 fis, clone TRACH2005210. AK124257 - Homo sapiens cDNA FLJ42263 fis, clone TKIDN2014570. LP057483 - Sequence 1 from Patent EP2841598. LP057484 - Sequence 2 from Patent EP2841598. LP057523 - Sequence 41 from Patent EP2841598. LP057524 - Sequence 42 from Patent EP2841598. HM437233 - Homo sapiens serine/threonine-protein kinase B-raf variant (BRAF) mRNA, partial cds. JD560335 - Sequence 541359 from Patent EP1572962. DJ444894 - Combinatorial Methods and Compositions for Treatment of Melanoma. AJ276310 - Homo sapiens partial mRNA for B-Raf protein (B-raf gene), alternative exon 10. AK054801 - Homo sapiens cDNA FLJ30239 fis, clone BRACE2002081, weakly similar to B-RAF PROTO-ONCOGENE SERINE/THREONINE-PROTEIN KINASE. JD019544 - Sequence 568 from Patent EP1572962. JD032247 - Sequence 13271 from Patent EP1572962.

Biochemical and Signaling Pathways


	KEGG - Kyoto Encyclopedia of Genes and Genomes hsa04010 - MAPK signaling pathway hsa04012 - ErbB signaling pathway hsa04062 - Chemokine signaling pathway hsa04150 - mTOR signaling pathway hsa04270 - Vascular smooth muscle contraction hsa04510 - Focal adhesion hsa04650 - Natural killer cell mediated cytotoxicity hsa04720 - Long-term potentiation hsa04722 - Neurotrophin signaling pathway hsa04730 - Long-term depression hsa04810 - Regulation of actin cytoskeleton hsa04910 - Insulin signaling pathway hsa04914 - Progesterone-mediated oocyte maturation hsa05200 - Pathways in cancer hsa05210 - Colorectal cancer hsa05211 - Renal cell carcinoma hsa05212 - Pancreatic cancer hsa05213 - Endometrial cancer hsa05214 - Glioma hsa05215 - Prostate cancer hsa05216 - Thyroid cancer hsa05218 - Melanoma hsa05219 - Bladder cancer hsa05220 - Chronic myeloid leukemia hsa05221 - Acute myeloid leukemia hsa05223 - Non-small cell lung cancer

Other Names for This Gene


	Alternate Gene Symbols: A0A2R8Y8E0, A0A2R8Y8E0_HUMAN, BRAF , ENST00000644969.1, NM_001374258, uc285zzl.1, uc285zzl.2 UCSC ID: ENST00000644969.2 RefSeq Accession: NM_001374258.1

GeneReviews for This Gene


	GeneReviews article(s) related to gene BRAF: cfc (Cardiofaciocutaneous Syndrome) leopard (Noonan Syndrome with Multiple Lentigines) noonan (Noonan Syndrome)

Methods, Credits, and Use Restrictions


	Click here for details on how this gene model was made and data restrictions if any.

GNF Expression Atlas 2 Data from U133A and GNF1H Chips