Human Gene CCL8 (ENST00000394620.2) Description and Page Index
  Description: Homo sapiens C-C motif chemokine ligand 8 (CCL8), mRNA. (from RefSeq NM_005623)
RefSeq Summary (NM_005623): This antimicrobial gene is one of several chemokine genes clustered on the q-arm of chromosome 17. Chemokines form a superfamily of secreted proteins involved in immunoregulatory and inflammatory processes. The superfamily is divided into four subfamilies based on the arrangement of N-terminal cysteine residues of the mature peptide. This chemokine is a member of the CC subfamily which is characterized by two adjacent cysteine residues. This cytokine displays chemotactic activity for monocytes, lymphocytes, basophils and eosinophils. By recruiting leukocytes to sites of inflammation this cytokine may contribute to tumor-associated leukocyte infiltration and to the antiviral state against HIV infection. [provided by RefSeq, Sep 2014]. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: Y16645.1, AV733664.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## CDS uses downstream in-frame AUG :: upstream AUG and CDS extension is not conserved MANE Ensembl match :: ENST00000394620.2/ ENSP00000378118.1 Protein has antimicrobial activity :: PMID: 12949249 RefSeq Select criteria :: based on single protein-coding transcript ##RefSeq-Attributes-END##
Gencode Transcript: ENST00000394620.2
Gencode Gene: ENSG00000108700.5
Transcript (Including UTRs)
   Position: hg38 chr17:34,319,435-34,321,402 Size: 1,968 Total Exon Count: 3 Strand: +
Coding Region
   Position: hg38 chr17:34,319,502-34,320,907 Size: 1,406 Coding Exon Count: 3 

Page IndexSequence and LinksUniProtKB CommentsMalaCardsCTDRNA-Seq Expression
Microarray ExpressionRNA StructureProtein StructureOther SpeciesGO AnnotationsmRNA Descriptions
PathwaysOther NamesMethods
Data last updated: 2019-09-04

-  Sequence and Links to Tools and Databases
 
Genomic Sequence (chr17:34,319,435-34,321,402)mRNA (may differ from genome)Protein (99 aa)
Gene SorterGenome BrowserOther Species FASTAGene interactionsTable SchemaBioGPS
CGAPEnsemblEntrez GeneExonPrimerGeneCardsHGNC
HPRDLynxMGIneXtProtOMIMPubMed
Stanford SOURCEUniProtKBWikipedia

-  Comments and Description Text from UniProtKB
  ID: CCL8_HUMAN
DESCRIPTION: RecName: Full=C-C motif chemokine 8; AltName: Full=HC14; AltName: Full=Monocyte chemoattractant protein 2; AltName: Full=Monocyte chemotactic protein 2; Short=MCP-2; AltName: Full=Small-inducible cytokine A8; Contains: RecName: Full=MCP-2(6-76); Flags: Precursor;
FUNCTION: Chemotactic factor that attracts monocytes, lymphocytes, basophils and eosinophils. May play a role in neoplasia and inflammatory host responses. This protein can bind heparin. The processed form MCP-2(6-76) does not show monocyte chemotactic activity, but inhibits the chemotactic effect most predominantly of CCL7, and also of CCL2 and CCL5 and CCL8.
SUBUNIT: Monomer or homodimer; in equilibrium.
SUBCELLULAR LOCATION: Secreted.
TISSUE SPECIFICITY: Highest expression found in the small intestine and peripheral blood cells. Intermediate levels seen in the heart, placenta, lung, skeletal muscle, thymus, colon, ovary, spinal cord and pancreas. Low levels seen in the brain, liver, spleen and prostate.
INDUCTION: By IFNG/IFN-gamma, mitogens and IL1/interleukin-1.
PTM: N-terminal processed form MCP-2(6-76) is produced by proteolytic cleavage after secretion from peripheral blood monocytes.
SIMILARITY: Belongs to the intercrine beta (chemokine CC) family.
SEQUENCE CAUTION: Sequence=CAA68168.1; Type=Erroneous initiation;
WEB RESOURCE: Name=Wikipedia; Note=CCL8 entry; URL="http://en.wikipedia.org/wiki/CCL8";

-  MalaCards Disease Associations
  MalaCards Gene Search: CCL8
Diseases sorted by gene-association score: diffuse gastric cancer (2), primary syphilis (1)

-  Comparative Toxicogenomics Database (CTD)
  The following chemicals interact with this gene           more ... click here to view the complete list

-  RNA-Seq Expression Data from GTEx (53 Tissues, 570 Donors)
  Highest median expression: 2.98 RPKM in Colon - Transverse
Total median expression: 19.94 RPKM



View in GTEx track of Genome Browser    View at GTEx portal     View GTEx Body Map

+  Microarray Expression Data
  Press "+" in the title bar above to open this section.

-  mRNA Secondary Structure of 3' and 5' UTRs
 
RegionFold EnergyBasesEnergy/Base
Display As
5' UTR -12.5067-0.187 Picture PostScript Text
3' UTR -105.90495-0.214 Picture PostScript Text

The RNAfold program from the Vienna RNA Package is used to perform the secondary structure predictions and folding calculations. The estimated folding energy is in kcal/mol. The more negative the energy, the more secondary structure the RNA is likely to have.

-  Protein Domain and Structure Information
  InterPro Domains: Graphical view of domain structure
IPR000827 - Chemokine_CC_CS
IPR001811 - Chemokine_IL8-like_dom

Pfam Domains:
PF00048 - Small cytokines (intecrine/chemokine), interleukin-8 like

Protein Data Bank (PDB) 3-D Structure
MuPIT help

1ESR
- X-ray MuPIT


ModBase Predicted Comparative 3D Structure on P80075
FrontTopSide
The pictures above may be empty if there is no ModBase structure for the protein. The ModBase structure frequently covers just a fragment of the protein. You may be asked to log onto ModBase the first time you click on the pictures. It is simplest after logging in to just click on the picture again to get to the specific info on that model.

-  Orthologous Genes in Other Species
  Orthologies between human, mouse, and rat are computed by taking the best BLASTP hit, and filtering out non-syntenic hits. For more distant species reciprocal-best BLASTP hits are used. Note that the absence of an ortholog in the table below may reflect incomplete annotations in the other species rather than a true absence of the orthologous gene.
MouseRatZebrafishD. melanogasterC. elegansS. cerevisiae
Genome BrowserGenome BrowserNo orthologNo orthologNo orthologNo ortholog
Gene Details     
Gene Sorter     
MGIRGD    
Protein SequenceProtein Sequence    
AlignmentAlignment    

-  Gene Ontology (GO) Annotations with Structured Vocabulary
  Molecular Function:
GO:0004672 protein kinase activity
GO:0005125 cytokine activity
GO:0008009 chemokine activity
GO:0008201 heparin binding
GO:0016004 phospholipase activator activity
GO:0048020 CCR chemokine receptor binding

Biological Process:
GO:0002548 monocyte chemotaxis
GO:0002687 positive regulation of leukocyte migration
GO:0006468 protein phosphorylation
GO:0006816 calcium ion transport
GO:0006874 cellular calcium ion homeostasis
GO:0006887 exocytosis
GO:0006935 chemotaxis
GO:0006954 inflammatory response
GO:0006955 immune response
GO:0007165 signal transduction
GO:0007186 G-protein coupled receptor signaling pathway
GO:0007267 cell-cell signaling
GO:0009615 response to virus
GO:0010469 regulation of receptor activity
GO:0030593 neutrophil chemotaxis
GO:0031640 killing of cells of other organism
GO:0043547 positive regulation of GTPase activity
GO:0048247 lymphocyte chemotaxis
GO:0070098 chemokine-mediated signaling pathway
GO:0070374 positive regulation of ERK1 and ERK2 cascade
GO:0070664 negative regulation of leukocyte proliferation
GO:0071346 cellular response to interferon-gamma
GO:0071347 cellular response to interleukin-1
GO:0071356 cellular response to tumor necrosis factor

Cellular Component:
GO:0005576 extracellular region
GO:0005615 extracellular space
GO:0005623 cell


-  Descriptions from all associated GenBank mRNAs
  KJ897524 - Synthetic construct Homo sapiens clone ccsbBroadEn_06918 CCL8 gene, encodes complete protein.
Y16645 - Homo sapiens mRNA for monocyte chemotactic protein-2.
MP067523 - Sequence 27 from Patent WO2018232288.
BC126242 - Homo sapiens chemokine (C-C motif) ligand 8, mRNA (cDNA clone MGC:161520 IMAGE:8991958), complete cds.
JD334412 - Sequence 315436 from Patent EP1572962.
JD186264 - Sequence 167288 from Patent EP1572962.
LQ878890 - Sequence 5 from Patent WO2018154075.
Y10802 - H.sapiens mRNA for monocyte chemotactic protein 2.
AB590535 - Synthetic construct DNA, clone: pFN21AB6759, Homo sapiens CCL8 gene for chemokine (C-C motif) ligand 8, without stop codon, in Flexi system.
HQ258700 - Synthetic construct Homo sapiens clone IMAGE:100072730 chemokine (C-C motif) ligand 8 (CCL8) (CCL8) gene, encodes complete protein.
JD290093 - Sequence 271117 from Patent EP1572962.
JD280859 - Sequence 261883 from Patent EP1572962.
JD498830 - Sequence 479854 from Patent EP1572962.
JD091638 - Sequence 72662 from Patent EP1572962.

-  Biochemical and Signaling Pathways
  KEGG - Kyoto Encyclopedia of Genes and Genomes
hsa04060 - Cytokine-cytokine receptor interaction
hsa04062 - Chemokine signaling pathway
hsa04621 - NOD-like receptor signaling pathway

-  Other Names for This Gene
  Alternate Gene Symbols: A0AV77, CCL8_HUMAN, MCP2, NM_005623, P78388, P80075, SCYA10, SCYA8, uc002hib.1, uc002hib.2, uc002hib.3, uc002hib.4, uc002hib.5
UCSC ID: uc002hib.5
RefSeq Accession: NM_005623
Protein: P80075 (aka CCL8_HUMAN)
CCDS: CCDS11280.1

-  Methods, Credits, and Use Restrictions
  Click here for details on how this gene model was made and data restrictions if any.