Human Gene CDC25B (ENST00000245960.10) from GENCODE V44
Description: Homo sapiens cell division cycle 25B (CDC25B), transcript variant 1, mRNA. (from RefSeq NM_021873) RefSeq Summary (NM_021873): CDC25B is a member of the CDC25 family of phosphatases. CDC25B activates the cyclin dependent kinase CDC2 by removing two phosphate groups and it is required for entry into mitosis. CDC25B shuttles between the nucleus and the cytoplasm due to nuclear localization and nuclear export signals. The protein is nuclear in the M and G1 phases of the cell cycle and moves to the cytoplasm during S and G2. CDC25B has oncogenic properties, although its role in tumor formation has not been determined. Multiple transcript variants for this gene exist. [provided by RefSeq, Jul 2008]. Gencode Transcript: ENST00000245960.10 Gencode Gene: ENSG00000101224.18 Transcript (Including UTRs) Position: hg38 chr20:3,796,310-3,806,115 Size: 9,806 Total Exon Count: 16 Strand: + Coding Region Position: hg38 chr20:3,796,532-3,804,961 Size: 8,430 Coding Exon Count: 16
ID:MPIP2_HUMAN DESCRIPTION: RecName: Full=M-phase inducer phosphatase 2; EC=3.1.3.48; AltName: Full=Dual specificity phosphatase Cdc25B; FUNCTION: Tyrosine protein phosphatase which functions as a dosage-dependent inducer of mitotic progression. Required for G2/M phases of the cell cycle progression and abscission during cytokinesis in a ECT2-dependent manner. Directly dephosphorylates CDK1 and stimulates its kinase activity. The three isoforms seem to have a different level of activity. CATALYTIC ACTIVITY: Protein tyrosine phosphate + H(2)O = protein tyrosine + phosphate. ENZYME REGULATION: Stimulated by B-type cyclins. SUBUNIT: Interacts with MAPK14 and 14-3-3 proteins. SUBCELLULAR LOCATION: Cytoplasm, cytoskeleton, centrosome. Cytoplasm, cytoskeleton, spindle pole. PTM: Phosphorylated by BRSK1 in vitro. Phosphorylated by CHEK1, which inhibits the activity of this protein. Phosphorylation at Ser-353 by AURKA might locally participate in the control of the onset of mitosis. Phosphorylation by MELK at Ser-169 promotes localization to the centrosome and the spindle poles during mitosis. Phosphorylation at Ser-323 and Ser-375 by MAPK14 is required for binding to 14-3-3 proteins. SIMILARITY: Belongs to the MPI phosphatase family. SIMILARITY: Contains 1 rhodanese domain. WEB RESOURCE: Name=NIEHS-SNPs; URL="http://egp.gs.washington.edu/data/cdc25b/";
The RNAfold program from the Vienna RNA Package is used to perform the secondary structure predictions and folding calculations. The estimated folding energy is in kcal/mol. The more negative the energy, the more secondary structure the RNA is likely to have.
ModBase Predicted Comparative 3D Structure on P30305
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Orthologous Genes in Other Species
Orthologies between human, mouse, and rat are computed by taking the best BLASTP hit, and filtering out non-syntenic hits. For more distant species reciprocal-best BLASTP hits are used. Note that the absence of an ortholog in the table below may reflect incomplete annotations in the other species rather than a true absence of the orthologous gene.
AK295573 - Homo sapiens cDNA FLJ53333 complete cds, highly similar to M-phase inducer phosphatase 2 (EC 3.1.3.48). AK299192 - Homo sapiens cDNA FLJ56140 complete cds, highly similar to M-phase inducer phosphatase 2 (EC 3.1.3.48). AK299028 - Homo sapiens cDNA FLJ55479 complete cds, highly similar to M-phase inducer phosphatase 2 (EC 3.1.3.48). AK092713 - Homo sapiens cDNA FLJ35394 fis, clone SKNSH2002768, highly similar to M-PHASE INDUCER PHOSPHATASE 2 (EC 3.1.3.48). FR695900 - Homo sapiens mRNA for isoform CDC25B6 (CDC25B gene). FR695901 - Homo sapiens mRNA for isoform CDC25B7 (CDC25B gene). BX647988 - Homo sapiens mRNA; cDNA DKFZp686N1752 (from clone DKFZp686N1752). BC051711 - Homo sapiens cell division cycle 25 homolog B (S. pombe), mRNA (cDNA clone MGC:44614 IMAGE:5301205), complete cds. S78187 - CDC25Hu2=cdc25+ homolog [human, mRNA, 3118 nt]. BC009953 - Homo sapiens cell division cycle 25 homolog B (S. pombe), mRNA (cDNA clone MGC:14269 IMAGE:4130804), complete cds. Z68092 - H.sapiens mRNA for tyrosine phosphatase. BC006395 - Homo sapiens cell division cycle 25 homolog B (S. pombe), mRNA (cDNA clone MGC:12797 IMAGE:4135465), complete cds. M81934 - Human cdc25B mRNA, complete cds. KJ890879 - Synthetic construct Homo sapiens clone ccsbBroadEn_00273 CDC25B gene, encodes complete protein. BX640836 - Homo sapiens mRNA; cDNA DKFZp686G14213 (from clone DKFZp686G14213). JD065989 - Sequence 47013 from Patent EP1572962. JD389850 - Sequence 370874 from Patent EP1572962. JD194242 - Sequence 175266 from Patent EP1572962. DL492376 - Novel nucleic acids. JD456935 - Sequence 437959 from Patent EP1572962. JD456936 - Sequence 437960 from Patent EP1572962. DL490864 - Novel nucleic acids. JD405826 - Sequence 386850 from Patent EP1572962. JD405870 - Sequence 386894 from Patent EP1572962. JD125348 - Sequence 106372 from Patent EP1572962. JD391573 - Sequence 372597 from Patent EP1572962. JD132313 - Sequence 113337 from Patent EP1572962. AK124284 - Homo sapiens cDNA FLJ42290 fis, clone TLIVE2007132. JD133729 - Sequence 114753 from Patent EP1572962. JD395818 - Sequence 376842 from Patent EP1572962. JD182891 - Sequence 163915 from Patent EP1572962. JD223839 - Sequence 204863 from Patent EP1572962. JD232565 - Sequence 213589 from Patent EP1572962. JD399819 - Sequence 380843 from Patent EP1572962. JD561720 - Sequence 542744 from Patent EP1572962. JD234260 - Sequence 215284 from Patent EP1572962. JD149248 - Sequence 130272 from Patent EP1572962. JD510182 - Sequence 491206 from Patent EP1572962. JD510419 - Sequence 491443 from Patent EP1572962. JD465732 - Sequence 446756 from Patent EP1572962. JD424793 - Sequence 405817 from Patent EP1572962. JD387464 - Sequence 368488 from Patent EP1572962. JD130488 - Sequence 111512 from Patent EP1572962. JD449626 - Sequence 430650 from Patent EP1572962. JD354421 - Sequence 335445 from Patent EP1572962. JD357467 - Sequence 338491 from Patent EP1572962. JD419842 - Sequence 400866 from Patent EP1572962.
Biochemical and Signaling Pathways
BioCarta from NCI Cancer Genome Anatomy Project h_cdc25Pathway - cdc25 and chk1 Regulatory Pathway in response to DNA damage h_rbPathway - RB Tumor Suppressor/Checkpoint Signaling in response to DNA damage h_g2Pathway - Cell Cycle: G2/M Checkpoint h_ptc1Pathway - Sonic Hedgehog (SHH) Receptor Ptc1 Regulates cell cycle h_srcRPTPPathway - Activation of Src by Protein-tyrosine phosphatase alpha
Reactome (by CSHL, EBI, and GO)
Protein P30305 (Reactome details) participates in the following event(s):
R-HSA-170120 Translocation of Cdc25B to the cytoplasm R-HSA-8863007 p25-bound CDK5 phosphorylates CDC25B R-HSA-170159 Translocation of Cdc25 to the nucleus R-HSA-170161 Dephosphorylation of cytoplasmic Cyclin B1/B2:phospho-Cdc2 (Thr 14, Tyr 15) complexes by CDC25B R-HSA-170153 Dephosphorylation of nuclear Cyclin B1:phospho-Cdc2 (Thr 14, Tyr15) complexes by Cdc25 phosphatases R-HSA-174110 Cdc25A mediated dephosphorylation of Cyclin A:phospho-Cdk2 R-HSA-69273 Cyclin A/B1/B2 associated events during G2/M transition R-HSA-8862803 Deregulated CDK5 triggers multiple neurodegenerative pathways in Alzheimer's disease models R-HSA-69275 G2/M Transition R-HSA-8863678 Neurodegenerative Diseases R-HSA-69656 Cyclin A:Cdk2-associated events at S phase entry R-HSA-453274 Mitotic G2-G2/M phases R-HSA-1643685 Disease R-HSA-69242 S Phase R-HSA-69278 Cell Cycle (Mitotic) R-HSA-1640170 Cell Cycle
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