Human Gene CENPH (ENST00000283006.7) from GENCODE V41
  Description: Homo sapiens centromere protein H (CENPH), mRNA. (from RefSeq NM_022909)
RefSeq Summary (NM_022909): Centromere and kinetochore proteins play a critical role in centromere structure, kinetochore formation, and sister chromatid separation. The protein encoded by this gene colocalizes with inner kinetochore plate proteins CENP-A and CENP-C in both interphase and metaphase. It localizes outside of centromeric heterochromatin, where CENP-B is localized, and inside the kinetochore corona, where CENP-E is localized during prometaphase. It is thought that this protein can bind to itself, as well as to CENP-A, CENP-B or CENP-C. Multimers of the protein localize constitutively to the inner kinetochore plate and play an important role in the organization and function of the active centromere-kinetochore complex. [provided by RefSeq, Jul 2008].
Gencode Transcript: ENST00000283006.7
Gencode Gene: ENSG00000153044.10
Transcript (Including UTRs)
   Position: hg38 chr5:69,189,583-69,210,357 Size: 20,775 Total Exon Count: 9 Strand: +
Coding Region
   Position: hg38 chr5:69,189,635-69,209,799 Size: 20,165 Coding Exon Count: 9 

Page IndexSequence and LinksUniProtKB CommentsCTDRNA-Seq ExpressionMicroarray Expression
RNA StructureProtein StructureOther SpeciesGO AnnotationsmRNA DescriptionsPathways
Other NamesMethods
Data last updated at UCSC: 2022-05-14 09:57:26

-  Sequence and Links to Tools and Databases
Genomic Sequence (chr5:69,189,583-69,210,357)mRNA (may differ from genome)Protein (247 aa)
Gene SorterGenome BrowserOther Species FASTAGene interactionsTable SchemaBioGPS
CGAPEnsemblEntrez GeneExonPrimerGencodeGeneCards

-  Comments and Description Text from UniProtKB
DESCRIPTION: RecName: Full=Centromere protein H; Short=CENP-H; AltName: Full=Interphase centromere complex protein 35;
FUNCTION: Component of the CENPA-NAC (nucleosome-associated) complex, a complex that plays a central role in assembly of kinetochore proteins, mitotic progression and chromosome segregation. The CENPA-NAC complex recruits the CENPA-CAD (nucleosome distal) complex and may be involved in incorporation of newly synthesized CENPA into centromeres. Required for chromosome congression and efficiently align the chromosomes on a metaphase plate.
SUBUNIT: Self-associates. Component of the CENPA-NAC complex, at least composed of CENPA, CENPC, CENPH, CENPM, CENPN, CENPT and MLF1IP/CENPU. The CENPA-NAC complex interacts with the CENPA-CAD complex, composed of CENPI, CENPK, CENPL, CENPO, CENPP, CENPQ, CENPR and CENPS. Interacts directly with CENPK. Interacts with KIF2C and NDC80. Interacts with TRIM36 (By similarity).
INTERACTION: O14777:NDC80; NbExp=3; IntAct=EBI-1003700, EBI-715849;
SUBCELLULAR LOCATION: Nucleus. Chromosome, centromere, kinetochore. Note=Associates with active centromere-kinetochore complexes throughout the cell cycle. Colocalizes with inner kinetochore plate proteins CENPA and CENPC1 during both interphase and metaphase.
SIMILARITY: Belongs to the centromere protein H family.

-  Comparative Toxicogenomics Database (CTD)
  The following chemicals interact with this gene           more ... click here to view the complete list

-  RNA-Seq Expression Data from GTEx (53 Tissues, 570 Donors)
  Highest median expression: 35.69 RPKM in Cells - EBV-transformed lymphocytes
Total median expression: 151.53 RPKM

View in GTEx track of Genome Browser    View at GTEx portal     View GTEx Body Map

+  Microarray Expression Data
  Press "+" in the title bar above to open this section.

-  mRNA Secondary Structure of 3' and 5' UTRs
RegionFold EnergyBasesEnergy/Base
Display As
5' UTR -12.5052-0.240 Picture PostScript Text
3' UTR -138.70558-0.249 Picture PostScript Text

The RNAfold program from the Vienna RNA Package is used to perform the secondary structure predictions and folding calculations. The estimated folding energy is in kcal/mol. The more negative the energy, the more secondary structure the RNA is likely to have.

-  Protein Domain and Structure Information
  InterPro Domains: Graphical view of domain structure
IPR008426 - Centromere_CenpH

Pfam Domains:
PF05837 - Centromere protein H (CENP-H)

ModBase Predicted Comparative 3D Structure on Q9H3R5
The pictures above may be empty if there is no ModBase structure for the protein. The ModBase structure frequently covers just a fragment of the protein. You may be asked to log onto ModBase the first time you click on the pictures. It is simplest after logging in to just click on the picture again to get to the specific info on that model.

-  Orthologous Genes in Other Species
  Orthologies between human, mouse, and rat are computed by taking the best BLASTP hit, and filtering out non-syntenic hits. For more distant species reciprocal-best BLASTP hits are used. Note that the absence of an ortholog in the table below may reflect incomplete annotations in the other species rather than a true absence of the orthologous gene.
MouseRatZebrafishD. melanogasterC. elegansS. cerevisiae
Genome BrowserGenome BrowserGenome BrowserNo orthologNo orthologNo ortholog
Gene Details     
Gene Sorter     
Protein SequenceProtein SequenceProtein Sequence   

-  Gene Ontology (GO) Annotations with Structured Vocabulary
  Molecular Function:
GO:0005515 protein binding
GO:0043515 kinetochore binding

Biological Process:
GO:0034080 CENP-A containing nucleosome assembly
GO:0051382 kinetochore assembly
GO:0051383 kinetochore organization

Cellular Component:
GO:0000775 chromosome, centromeric region
GO:0000776 kinetochore
GO:0000777 condensed chromosome kinetochore
GO:0005634 nucleus
GO:0005654 nucleoplasm
GO:0005694 chromosome
GO:0005730 nucleolus
GO:0005829 cytosol

-  Descriptions from all associated GenBank mRNAs
  AK290746 - Homo sapiens cDNA FLJ76140 complete cds, highly similar to Homo sapiens centromere protein H (CENPH), mRNA.
BC015355 - Homo sapiens centromere protein H, mRNA (cDNA clone MGC:21458 IMAGE:3451767), complete cds.
BC012024 - Homo sapiens centromere protein H, mRNA (cDNA clone MGC:21431 IMAGE:4510607), complete cds.
AK123761 - Homo sapiens cDNA FLJ41767 fis, clone IMR322006495, highly similar to Centromere protein H (Kinetochore protein CENP-H).
KU178758 - Homo sapiens centromere protein H isoform 1 (CENPH) mRNA, partial cds.
KU178759 - Homo sapiens centromere protein H isoform 2 (CENPH) mRNA, complete cds, alternatively spliced.
DQ894658 - Synthetic construct Homo sapiens clone IMAGE:100009118; FLH177277.01L; RZPDo839F08123D centromere protein H (CENPH) gene, encodes complete protein.
AB035124 - Homo sapiens mRNA for kinetochore protein CENP-H, complete cds.
KJ894593 - Synthetic construct Homo sapiens clone ccsbBroadEn_03987 CENPH gene, encodes complete protein.
CU680168 - Synthetic construct Homo sapiens gateway clone IMAGE:100016885 5' read CENPH mRNA.
JD040889 - Sequence 21913 from Patent EP1572962.
DQ587113 - Homo sapiens piRNA piR-54225, complete sequence.
JD385540 - Sequence 366564 from Patent EP1572962.
JD564604 - Sequence 545628 from Patent EP1572962.
JD564603 - Sequence 545627 from Patent EP1572962.
JD531657 - Sequence 512681 from Patent EP1572962.
JD396176 - Sequence 377200 from Patent EP1572962.
JD409266 - Sequence 390290 from Patent EP1572962.
JD489082 - Sequence 470106 from Patent EP1572962.
JD309789 - Sequence 290813 from Patent EP1572962.
JD070002 - Sequence 51026 from Patent EP1572962.
JD502842 - Sequence 483866 from Patent EP1572962.
JD479922 - Sequence 460946 from Patent EP1572962.
JD200677 - Sequence 181701 from Patent EP1572962.
JD476477 - Sequence 457501 from Patent EP1572962.
JD411595 - Sequence 392619 from Patent EP1572962.

-  Biochemical and Signaling Pathways
  Reactome (by CSHL, EBI, and GO)

Protein Q9H3R5 (Reactome details) participates in the following event(s):

R-HSA-606349 Mis18 complex binds the centromere
R-HSA-141409 Mad1 binds kinetochore
R-HSA-375302 Kinetochore capture of astral microtubules
R-HSA-5666129 CDC42:GTP recruits DIAPH2-2 to kinetochores
R-HSA-5666169 Kinetochore capture of astral microtubules is positively regulated by CDC42:GTP:p-S196-DIAPH2-2
R-HSA-606326 HJURP:CENPA complex localizes to the centromere
R-HSA-141431 MAD2 associates with the Mad1 kinetochore complex
R-HSA-141439 Release of activated MAD2 from kinetochores
R-HSA-2467811 Separation of sister chromatids
R-HSA-2467809 ESPL1 (Separase) cleaves centromeric cohesin
R-HSA-5666160 AURKB phosphorylates DIAPH2-2 at kinetochores
R-HSA-141422 MAD2 converted to an inhibitory state via interaction with Mad1
R-HSA-1638821 PP2A-B56 dephosphorylates centromeric cohesin
R-HSA-1638803 Phosphorylation of cohesin by PLK1 at centromeres
R-HSA-2468287 CDK1 phosphorylates CDCA5 (Sororin) at centromeres
R-HSA-606279 Deposition of new CENPA-containing nucleosomes at the centromere
R-HSA-141444 Amplification of signal from unattached kinetochores via a MAD2 inhibitory signal
R-HSA-68877 Mitotic Prometaphase
R-HSA-5663220 RHO GTPases Activate Formins
R-HSA-774815 Nucleosome assembly
R-HSA-2500257 Resolution of Sister Chromatid Cohesion
R-HSA-2467813 Separation of Sister Chromatids
R-HSA-141424 Amplification of signal from the kinetochores
R-HSA-68886 M Phase
R-HSA-195258 RHO GTPase Effectors
R-HSA-73886 Chromosome Maintenance
R-HSA-68882 Mitotic Anaphase
R-HSA-69618 Mitotic Spindle Checkpoint
R-HSA-69278 Cell Cycle (Mitotic)
R-HSA-194315 Signaling by Rho GTPases
R-HSA-1640170 Cell Cycle
R-HSA-2555396 Mitotic Metaphase and Anaphase
R-HSA-69620 Cell Cycle Checkpoints
R-HSA-162582 Signal Transduction

-  Other Names for This Gene
  Alternate Gene Symbols: A8K3Y1, CENPH_HUMAN, ENST00000283006.1, ENST00000283006.2, ENST00000283006.3, ENST00000283006.4, ENST00000283006.5, ENST00000283006.6, ICEN35, NM_022909, Q9H3R5, uc003jvp.1, uc003jvp.2, uc003jvp.3, uc003jvp.4
UCSC ID: ENST00000283006.7
RefSeq Accession: NM_022909
Protein: Q9H3R5 (aka CENPH_HUMAN)
CCDS: CCDS3998.1

-  Methods, Credits, and Use Restrictions
  Click here for details on how this gene model was made and data restrictions if any.