Human Gene CHRNB4 (ENST00000261751.8) from GENCODE V49

JavaScript is disabled in your web browser

You must have JavaScript enabled in your web browser to use the Genome Browser

Description: cholinergic receptor nicotinic beta 4 subunit, transcript variant 1 (from RefSeq NM_000750.5)
Gencode Transcript: ENST00000261751.8
Gencode Gene: ENSG00000117971.13
Transcript (Including UTRs)
Position: hg38 chr15:78,624,111-78,641,210 Size: 17,100 Total Exon Count: 6 Strand: -
Coding Region
Position: hg38 chr15:78,625,133-78,641,133 Size: 16,001 Coding Exon Count: 6

Page Index	Sequence and Links	UniProtKB Comments	Primers	MalaCards	CTD
RNA-Seq Expression	Microarray Expression	RNA Structure	Protein Structure	Other Species	GO Annotations
mRNA Descriptions	Pathways	Other Names	Methods

Data last updated at UCSC: 2025-09-17 13:57:07

Sequence and Links to Tools and Databases

Genomic Sequence (chr15:78,624,111-78,641,210)			mRNA (may differ from genome)		Protein (498 aa)
Gene Sorter	Genome Browser	Other Species FASTA	Gene interactions	Table Schema	AlphaFold
BioGPS	Ensembl	Entrez Gene	ExonPrimer	Gencode	GeneCards
HGNC	Lynx	Malacards	MGI	OMIM	PubMed
Reactome	UniProtKB	Wikipedia	BioGrid CRISPR DB

Comments and Description Text from UniProtKB


	ID: ACHB4_HUMAN DESCRIPTION: RecName: Full=Neuronal acetylcholine receptor subunit beta-4; Flags: Precursor; FUNCTION: After binding acetylcholine, the AChR responds by an extensive change in conformation that affects all subunits and leads to opening of an ion-conducting channel across the plasma membrane. SUBUNIT: Neuronal AChR is composed of two different types of subunits: alpha and beta. Beta-4 subunit can be combined to alpha- 2, alpha-3 or alpha-4 to give rise to functional receptors. Interacts with RIC3; which is required for proper folding and assembly. SUBCELLULAR LOCATION: Cell junction, synapse, postsynaptic cell membrane; Multi-pass membrane protein. Cell membrane; Multi-pass membrane protein. SIMILARITY: Belongs to the ligand-gated ion channel (TC 1.A.9) family. Acetylcholine receptor (TC 1.A.9.1) subfamily. Beta- 4/CHRNB4 sub-subfamily.

Primer design for this transcript

Primer3Plus can design qPCR Primers that straddle exon-exon-junctions, which amplify only cDNA, not genomic DNA.
Click here to load the transcript sequence and exon structure into Primer3Plus

Exonprimer can design one pair of Sanger sequencing primers around every exon, located in non-genic sequence.
Click here to open Exonprimer with this transcript

To design primers for a non-coding sequence, zoom to a region of interest and select from the drop-down menu: View > In External Tools > Primer3

MalaCards Disease Associations


	MalaCards Gene Search: CHRNB4 Diseases sorted by gene-association score: substance dependence (8), nicotine dependence, protection against (5), lung cancer (2)

Comparative Toxicogenomics Database (CTD)


	The following chemicals interact with this gene D009538 Nicotine D000109 Acetylcholine D014403 Tubocurarine C082748 epibatidine D002217 Carbachol D004079 Dihydro-beta-Erythroidine D004246 Dimethylphenylpiperazinium Iodide D018738 Hexamethonium D008464 Mecamylamine C004712 cytisine more ... click here to view the complete list

RNA-Seq Expression Data from GTEx (53 Tissues, 570 Donors)


	Highest median expression: 4.62 RPKM in Testis Total median expression: 6.80 RPKM View in GTEx track of Genome Browser View at GTEx portal View GTEx Body Map

Microarray Expression Data


	Press "+" in the title bar above to open this section.

mRNA Secondary Structure of 3' and 5' UTRs

Region	Fold Energy	Bases	Energy/Base	Display As
5' UTR	-36.80	77	-0.478	Picture	PostScript	Text
3' UTR	-331.30	1022	-0.324	Picture	PostScript	Text

The RNAfold program from the Vienna RNA Package is used to perform the secondary structure predictions and folding calculations. The estimated folding energy is in kcal/mol. The more negative the energy, the more secondary structure the RNA is likely to have.

Protein Domain and Structure Information

InterPro Domains: Graphical view of domain structure
IPR006202 - Neur_chan_lig-bd
IPR006201 - Neur_channel
IPR006029 - Neurotrans-gated_channel_TM
IPR018000 - Neurotransmitter_ion_chnl_CS
IPR002394 - Nicotinic_acetylcholine_rcpt

Pfam Domains:
PF02931 - Neurotransmitter-gated ion-channel ligand binding domain
PF02932 - Neurotransmitter-gated ion-channel transmembrane region

Protein Data Bank (PDB) 3-D Structure
MuPIT help

2ASG - Model

ModBase Predicted Comparative 3D Structure on P30926


Front	Top	Side

The pictures above may be empty if there is no ModBase structure for the protein. The ModBase structure frequently covers just a fragment of the protein. You may be asked to log onto ModBase the first time you click on the pictures. It is simplest after logging in to just click on the picture again to get to the specific info on that model.

Orthologous Genes in Other Species

Orthologies between human, mouse, and rat are computed by taking the best BLASTP hit, and filtering out non-syntenic hits. For more distant species reciprocal-best BLASTP hits are used. Note that the absence of an ortholog in the table below may reflect incomplete annotations in the other species rather than a true absence of the orthologous gene.

Mouse	Rat	Zebrafish	D. melanogaster	C. elegans	S. cerevisiae
Genome Browser	Genome Browser	No ortholog	No ortholog	No ortholog	No ortholog
Gene Details
Gene Sorter
MGI	RGD
Protein Sequence	Protein Sequence
Alignment	Alignment

Gene Ontology (GO) Annotations with Structured Vocabulary


	Molecular Function: GO:0004888 transmembrane signaling receptor activity GO:0005216 ion channel activity GO:0005230 extracellular ligand-gated ion channel activity GO:0005515 protein binding GO:0008144 drug binding GO:0015276 ligand-gated ion channel activity GO:0015464 acetylcholine receptor activity GO:0022848 acetylcholine-gated cation-selective channel activity GO:0042166 acetylcholine binding GO:0046982 protein heterodimerization activity Biological Process: GO:0001508 action potential GO:0006811 ion transport GO:0006939 smooth muscle contraction GO:0006940 regulation of smooth muscle contraction GO:0007165 signal transduction GO:0007271 synaptic transmission, cholinergic GO:0007626 locomotory behavior GO:0034220 ion transmembrane transport GO:0035094 response to nicotine GO:0035095 behavioral response to nicotine GO:0042391 regulation of membrane potential GO:0043312 neutrophil degranulation GO:0046928 regulation of neurotransmitter secretion GO:0051291 protein heterooligomerization GO:0051971 positive regulation of transmission of nerve impulse GO:0060078 regulation of postsynaptic membrane potential GO:0060079 excitatory postsynaptic potential GO:0060084 synaptic transmission involved in micturition Cellular Component: GO:0005886 plasma membrane GO:0005887 integral component of plasma membrane GO:0005892 acetylcholine-gated channel complex GO:0016020 membrane GO:0016021 integral component of membrane GO:0030054 cell junction GO:0035579 specific granule membrane GO:0043005 neuron projection GO:0045202 synapse GO:0045211 postsynaptic membrane GO:0070821 tertiary granule membrane

Descriptions from all associated GenBank mRNAs


	AK310443 - Homo sapiens cDNA, FLJ17485. U48861 - Human beta 4 nicotinic acetylcholine receptor subunit mRNA, complete cds. JD148801 - Sequence 129825 from Patent EP1572962. JD108861 - Sequence 89885 from Patent EP1572962. JD163621 - Sequence 144645 from Patent EP1572962. JD164071 - Sequence 145095 from Patent EP1572962. JD164070 - Sequence 145094 from Patent EP1572962. JD374407 - Sequence 355431 from Patent EP1572962. JD345558 - Sequence 326582 from Patent EP1572962. JD284082 - Sequence 265106 from Patent EP1572962. JD521225 - Sequence 502249 from Patent EP1572962. JD353269 - Sequence 334293 from Patent EP1572962. JD193263 - Sequence 174287 from Patent EP1572962. U62439 - Human nicotinic acetylcholine receptor beta4 subunit precursor, mRNA, complete cds. JD530932 - Sequence 511956 from Patent EP1572962. JD327663 - Sequence 308687 from Patent EP1572962. BC096080 - Homo sapiens cholinergic receptor, nicotinic, beta 4, mRNA (cDNA clone MGC:116718 IMAGE:40000733), complete cds. BC096081 - Homo sapiens cholinergic receptor, nicotinic, beta 4, mRNA (cDNA clone IMAGE:40000736), partial cds. BC096082 - Homo sapiens cholinergic receptor, nicotinic, beta 4, mRNA (cDNA clone MGC:116720 IMAGE:40000737), complete cds. BC096083 - Homo sapiens cholinergic receptor, nicotinic, beta 4, mRNA (cDNA clone IMAGE:40000738), partial cds. JD490055 - Sequence 471079 from Patent EP1572962. JD221136 - Sequence 202160 from Patent EP1572962. X68275 - H.sapiens mRNA for nicotinic receptor beta 4 subunit. Y08416 - H.sapiens mRNA for nicotinic acetylcholine receptor beta4 subunit precursor. KJ890916 - Synthetic construct Homo sapiens clone ccsbBroadEn_00310 CHRNB4 gene, encodes complete protein. KR711747 - Synthetic construct Homo sapiens clone CCSBHm_00030749 CHRNB4 (CHRNB4) mRNA, encodes complete protein. KR711748 - Synthetic construct Homo sapiens clone CCSBHm_00030750 CHRNB4 (CHRNB4) mRNA, encodes complete protein. KR711749 - Synthetic construct Homo sapiens clone CCSBHm_00030751 CHRNB4 (CHRNB4) mRNA, encodes complete protein. DQ588795 - Homo sapiens piRNA piR-55907, complete sequence. DQ583701 - Homo sapiens piRNA piR-50813, complete sequence. JD247153 - Sequence 228177 from Patent EP1572962.

Biochemical and Signaling Pathways


	KEGG - Kyoto Encyclopedia of Genes and Genomes hsa04080 - Neuroactive ligand-receptor interaction Reactome (by CSHL, EBI, and GO) Protein P30926 (Reactome details) participates in the following event(s): R-HSA-6799350 Exocytosis of specific granule membrane proteins R-HSA-6798747 Exocytosis of tertiary granule membrane proteins R-HSA-622325 Activation of highly sodium permeable nicotinic acetylcholine receptors R-HSA-629588 Binding of acetylcholine to highly sodium permeable acetylcholine receptors R-HSA-622326 Activation of highly calcium permeable nicotinic acetylcholine receptors R-HSA-629599 Binding of acetylcholine to highly calcium permeable acetylcholine receptors R-HSA-629595 Activation of highly calcium permeable postsynaptic nicotinic acetylcholine receptors R-HSA-629596 Binding of acetylcholine to highly calcium permeable postsynaptic nicotinic acetylcholine receptors R-HSA-6798695 Neutrophil degranulation R-HSA-629587 Highly sodium permeable acetylcholine nicotinic receptors R-HSA-629597 Highly calcium permeable nicotinic acetylcholine receptors R-HSA-168249 Innate Immune System R-HSA-622323 Presynaptic nicotinic acetylcholine receptors R-HSA-622327 Postsynaptic nicotinic acetylcholine receptors R-HSA-629594 Highly calcium permeable postsynaptic nicotinic acetylcholine receptors R-HSA-168256 Immune System R-HSA-629602 Activation of Nicotinic Acetylcholine Receptors R-HSA-181431 Acetylcholine binding and downstream events R-HSA-112314 Neurotransmitter receptors and postsynaptic signal transmission R-HSA-112315 Transmission across Chemical Synapses R-HSA-112316 Neuronal System

Other Names for This Gene


	Alternate Gene Symbols: A4FTX5, ACHB4_HUMAN, E9PHE8, ENST00000261751.1, ENST00000261751.2, ENST00000261751.3, ENST00000261751.4, ENST00000261751.5, ENST00000261751.6, ENST00000261751.7, NM_000750, P30926, Q16607, Q4VBA5, Q8WXC8, Q9BQR4, uc002bed.1, uc002bed.2, uc002bed.3 UCSC ID: ENST00000261751.8 RefSeq Accession: NM_000750.5 Protein: P30926 (aka ACHB4_HUMAN or ACHP_HUMAN) CCDS: CCDS10306.1

Methods, Credits, and Use Restrictions


	Click here for details on how this gene model was made and data restrictions if any.