Human Gene CYP3A43 (ENST00000354829.6) Description and Page Index
  Description: Homo sapiens cytochrome P450 family 3 subfamily A member 43 (CYP3A43), transcript variant 6, non-coding RNA. (from RefSeq NR_103869)
RefSeq Summary (NM_057095): This gene encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. The encoded protein has a low level of testosterone hydroxylase activity, and may play a role in aging mechanisms and cancer progression. This gene is part of a cluster of cytochrome P450 genes on chromosome 7q21.1. Alternate splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Jul 2013].
Gencode Transcript: ENST00000354829.6
Gencode Gene: ENSG00000021461.16
Transcript (Including UTRs)
   Position: hg38 chr7:99,828,013-99,866,095 Size: 38,083 Total Exon Count: 13 Strand: +
Coding Region
   Position: hg38 chr7:99,828,116-99,866,001 Size: 37,886 Coding Exon Count: 13 

Page IndexSequence and LinksUniProtKB CommentsCTDRNA-Seq ExpressionMicroarray Expression
RNA StructureProtein StructureOther SpeciesGO AnnotationsmRNA DescriptionsPathways
Other NamesMethods
Data last updated: 2019-09-04

-  Sequence and Links to Tools and Databases
 
Genomic Sequence (chr7:99,828,013-99,866,095)mRNA (may differ from genome)Protein (503 aa)
Gene SorterGenome BrowserOther Species FASTAGene interactionsTable SchemaBioGPS
CGAPEnsemblEntrez GeneExonPrimerGeneCardsHGNC
LynxMGIneXtProtOMIMPubMedReactome
Stanford SOURCEUniProtKBWikipedia

-  Comments and Description Text from UniProtKB
  ID: CP343_HUMAN
DESCRIPTION: RecName: Full=Cytochrome P450 3A43; EC=1.14.14.1;
FUNCTION: Exhibits low testosterone 6-beta-hydroxylase activity.
CATALYTIC ACTIVITY: RH + reduced flavoprotein + O(2) = ROH + oxidized flavoprotein + H(2)O.
COFACTOR: Heme group (By similarity).
SUBCELLULAR LOCATION: Endoplasmic reticulum membrane; Peripheral membrane protein. Microsome membrane; Peripheral membrane protein.
TISSUE SPECIFICITY: Highest expression level in prostate. Also expressed in liver, kidney, pancreas, fetal liver and fetal skeletal muscle.
INDUCTION: By rifampicin.
POLYMORPHISM: At protein level, three alleles are known: CYP3A43*1, CYP3A43*2 and CYP3A43*3. The sequence shown is that of CYP3A43*1, which is the most frequent allele. The allele CYP3A43*2 is likely to be non-functional.
MISCELLANEOUS: Chimeric transcripts, characterized by CYP3A43 exon 1 joined at canonical splice sites to distinct sets of CYP3A4 or CYP3A5 exons, have been detected. All are possibly produced by trans-splicing. CYP3A43-CYP3A4 chimeric transcripts exist in 3 different combinations: CYP3A43 exon 1 joined in frame to CYP3A4 exons 2-13, CYP3A43 exon 1 joined in frame to CYP3A4 exons 4-13 and CYP3A43 exon 1 joined in frame to CYP3A4 exon 7-13. The longest chimeric isoform (CYP3A43 exon 1 joined to CYP3A4 exons 2- 13) exhibits 6-beta-hydroxylase activity, while a shorter isoform (CYP3A43 exon 1 joined to CYP3A4 exons 4-13) does not. CYP3A43- CYP3A5 chimeric transcripts exist in 2 different combinations: CYP3A43 exon 1 joined in frame to CYP3A5 exon 11-13 and CYP3A43 exon 1 joined in frame to CYP3A5 exon 12-13. All chimeric transcripts are expressed at very low levels in the liver (PubMed:11726664).
SIMILARITY: Belongs to the cytochrome P450 family.
WEB RESOURCE: Name=Cytochrome P450 Allele Nomenclature Committee; Note=CYP3A43 alleles; URL="http://www.cypalleles.ki.se/cyp3a43.htm";

-  Comparative Toxicogenomics Database (CTD)
  The following chemicals interact with this gene

-  RNA-Seq Expression Data from GTEx (53 Tissues, 570 Donors)
  Highest median expression: 2.36 RPKM in Liver
Total median expression: 6.98 RPKM



View in GTEx track of Genome Browser    View at GTEx portal     View GTEx Body Map

+  Microarray Expression Data
  Press "+" in the title bar above to open this section.

-  mRNA Secondary Structure of 3' and 5' UTRs
 
RegionFold EnergyBasesEnergy/Base
Display As
5' UTR -19.70103-0.191 Picture PostScript Text
3' UTR -10.0094-0.106 Picture PostScript Text

The RNAfold program from the Vienna RNA Package is used to perform the secondary structure predictions and folding calculations. The estimated folding energy is in kcal/mol. The more negative the energy, the more secondary structure the RNA is likely to have.

-  Protein Domain and Structure Information
  InterPro Domains: Graphical view of domain structure
IPR001128 - Cyt_P450
IPR017972 - Cyt_P450_CS
IPR008072 - Cyt_P450_E_CYP3A
IPR002402 - Cyt_P450_E_grp-II

Pfam Domains:
PF00067 - Cytochrome P450

ModBase Predicted Comparative 3D Structure on Q9HB55
FrontTopSide
The pictures above may be empty if there is no ModBase structure for the protein. The ModBase structure frequently covers just a fragment of the protein. You may be asked to log onto ModBase the first time you click on the pictures. It is simplest after logging in to just click on the picture again to get to the specific info on that model.

-  Orthologous Genes in Other Species
  Orthologies between human, mouse, and rat are computed by taking the best BLASTP hit, and filtering out non-syntenic hits. For more distant species reciprocal-best BLASTP hits are used. Note that the absence of an ortholog in the table below may reflect incomplete annotations in the other species rather than a true absence of the orthologous gene.
MouseRatZebrafishD. melanogasterC. elegansS. cerevisiae
Genome BrowserGenome BrowserNo orthologNo orthologNo orthologNo ortholog
Gene Details     
Gene Sorter     
MGIRGD    
Protein SequenceProtein Sequence    
AlignmentAlignment    

-  Gene Ontology (GO) Annotations with Structured Vocabulary
  Molecular Function:
GO:0004497 monooxygenase activity
GO:0005506 iron ion binding
GO:0016491 oxidoreductase activity
GO:0016705 oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen
GO:0016712 oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen, reduced flavin or flavoprotein as one donor, and incorporation of one atom of oxygen
GO:0020037 heme binding
GO:0046872 metal ion binding
GO:0070330 aromatase activity

Biological Process:
GO:0055114 oxidation-reduction process

Cellular Component:
GO:0005783 endoplasmic reticulum
GO:0005789 endoplasmic reticulum membrane
GO:0016020 membrane
GO:0031090 organelle membrane
GO:0043231 intracellular membrane-bounded organelle


-  Descriptions from all associated GenBank mRNAs
  JD322301 - Sequence 303325 from Patent EP1572962.
AF337813 - Homo sapiens cytochrome P450 subfamily IIIA polypeptide 43 (CYP3A43) mRNA, complete cds.
JD152716 - Sequence 133740 from Patent EP1572962.
JD338244 - Sequence 319268 from Patent EP1572962.
BC100981 - Homo sapiens cytochrome P450, family 3, subfamily A, polypeptide 43, mRNA (cDNA clone MGC:119315 IMAGE:40005401), complete cds.
BC100982 - Homo sapiens cytochrome P450, family 3, subfamily A, polypeptide 43, mRNA (cDNA clone MGC:119316 IMAGE:40005402), complete cds.
JD098267 - Sequence 79291 from Patent EP1572962.
JD307918 - Sequence 288942 from Patent EP1572962.
KJ903103 - Synthetic construct Homo sapiens clone ccsbBroadEn_12497 CYP3A43 gene, encodes complete protein.
AF280108 - Homo sapiens clone 15e cytochrome P450 subfamily IIIA polypeptide 43 (CYP3A43) mRNA, complete cds.
AF280109 - Homo sapiens clone 15a cytochrome P450 subfamily IIIA polypeptide 43 (CYP3A43) mRNA, complete cds, alternatively spliced.
AF280110 - Homo sapiens clone 15b cytochrome P450 subfamily IIIA polypeptide 43 (CYP3A43) mRNA, complete cds, alternatively spliced.
AF280111 - Homo sapiens clone 15d cytochrome P450 subfamily IIIA polypeptide 43 (CYP3A43) mRNA, complete cds, alternatively spliced.
AF280112 - Homo sapiens clone 15c cytochrome P450 subfamily IIIA polypeptide 43 (CYP3A43) mRNA, complete cds, alternatively spliced.
AF280113 - Homo sapiens clone 15g cytochrome P450 subfamily IIIA polypeptide 43 (CYP3A43) mRNA, complete cds, alternatively spliced.
AF319634 - Homo sapiens cytochrome P450 CYP3A43 (CYP3A43) mRNA, complete cds.
AY390423 - Homo sapiens nonfunctional cytochrome P450 CYP3A43.2 (CYP3A43) mRNA, CYP3A43*2 allele, complete cds.
AY390424 - Homo sapiens cytochrome P450 CYP3A43.3 (CYP3A43) mRNA, CYP3A43*3 allele, complete cds.
AY390425 - Homo sapiens cytochrome P450 CYP3A43.1 (CYP3A43) mRNA, CYP3A43*1B allele, complete cds.
AY390426 - Homo sapiens nonfunctional cytochrome P450 CYP3A43.2 (CYP3A43) mRNA, CYP3A43*2B allele, complete cds.
JD258284 - Sequence 239308 from Patent EP1572962.
JD281001 - Sequence 262025 from Patent EP1572962.

-  Biochemical and Signaling Pathways
  KEGG - Kyoto Encyclopedia of Genes and Genomes
hsa00140 - Steroid hormone biosynthesis
hsa00591 - Linoleic acid metabolism
hsa00830 - Retinol metabolism
hsa00980 - Metabolism of xenobiotics by cytochrome P450
hsa00982 - Drug metabolism - cytochrome P450
hsa00983 - Drug metabolism - other enzymes
hsa01100 - Metabolic pathways

Reactome (by CSHL, EBI, and GO)

Protein Q9HB55 (Reactome details) participates in the following event(s):

R-HSA-211959 CYP3A43 6b-hydroxylates TEST
R-HSA-213175 CYP3A4,5 oxidise AFB1 to AFXBO
R-HSA-211882 CYP3A7 can 6beta-hydroxylate testosterone
R-HSA-211948 CYP3A4 can N-demethylate loperamide
R-HSA-211958 Miscellaneous substrates
R-HSA-211981 Xenobiotics
R-HSA-211897 Cytochrome P450 - arranged by substrate type
R-HSA-211945 Phase I - Functionalization of compounds
R-HSA-211859 Biological oxidations
R-HSA-1430728 Metabolism

-  Other Names for This Gene
  Alternate Gene Symbols: CP343_HUMAN, NR_103869, Q75MK2, Q9HB52, Q9HB53, Q9HB54, Q9HB55, Q9HB57, uc003urx.1, uc003urx.2, uc003urx.3
UCSC ID: uc003urx.3
RefSeq Accession: NM_057095
Protein: Q9HB55 (aka CP343_HUMAN or C343_HUMAN)
CCDS: CCDS5675.1, CCDS5676.1

-  Methods, Credits, and Use Restrictions
  Click here for details on how this gene model was made and data restrictions if any.