Human Gene ADPGK (ENST00000456471.3) from GENCODE V44
  Description: Homo sapiens ADP dependent glucokinase (ADPGK), transcript variant 8, mRNA. (from RefSeq NM_001365229)
RefSeq Summary (NM_001365225): ADPGK (EC 2.7.1.147) catalyzes the ADP-dependent phosphorylation of glucose to glucose-6-phosphate and may play a role in glycolysis, possibly during ischemic conditions (Ronimus and Morgan, 2004 [PubMed 14975750]).[supplied by OMIM, Mar 2008]. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.
Gencode Transcript: ENST00000456471.3
Gencode Gene: ENSG00000159322.18
Transcript (Including UTRs)
   Position: hg38 chr15:72,751,294-72,783,758 Size: 32,465 Total Exon Count: 7 Strand: -
Coding Region
   Position: hg38 chr15:72,752,341-72,783,691 Size: 31,351 Coding Exon Count: 7 

Page IndexSequence and LinksUniProtKB CommentsPrimersMalaCardsCTD
RNA-Seq ExpressionMicroarray ExpressionRNA StructureProtein StructureOther SpeciesGO Annotations
mRNA DescriptionsPathwaysOther NamesMethods
Data last updated at UCSC: 2023-08-18 00:09:47

-  Sequence and Links to Tools and Databases
 
Genomic Sequence (chr15:72,751,294-72,783,758)mRNA (may differ from genome)Protein (497 aa)
Gene SorterGenome BrowserOther Species FASTAGene interactionsTable SchemaAlphaFold
BioGPSEnsemblEntrez GeneExonPrimerGencodeGeneCards
HGNCHPRDMalacardsMGIneXtProtOMIM
PubMedReactomeUniProtKBWikipediaBioGrid CRISPR DB

-  Comments and Description Text from UniProtKB
  ID: ADPGK_HUMAN
DESCRIPTION: RecName: Full=ADP-dependent glucokinase; Short=ADP-GK; Short=ADPGK; EC=2.7.1.147; AltName: Full=RbBP-35; Flags: Precursor;
FUNCTION: Catalyzes the phosphorylation of D-glucose to D-glucose 6-phosphate using ADP as the phosphate donor. GDP and CDP can replace ADP, but with reduced efficiency (By similarity).
CATALYTIC ACTIVITY: ADP + D-glucose = AMP + D-glucose 6-phosphate.
COFACTOR: Binds 1 magnesium ion per subunit (By similarity).
PATHWAY: Carbohydrate degradation; glycolysis.
SUBUNIT: Monomer (By similarity).
SUBCELLULAR LOCATION: Secreted (Potential).
SIMILARITY: Belongs to the ADP-dependent glucokinase family.
SIMILARITY: Contains 1 ADPK (ADP-dependent kinase) domain.

-  Primer design for this transcript
 

Primer3Plus can design qPCR Primers that straddle exon-exon-junctions, which amplify only cDNA, not genomic DNA.
Click here to load the transcript sequence and exon structure into Primer3Plus

Exonprimer can design one pair of Sanger sequencing primers around every exon, located in non-genic sequence.
Click here to open Exonprimer with this transcript

To design primers for a non-coding sequence, zoom to a region of interest and select from the drop-down menu: View > In External Tools > Primer3


-  MalaCards Disease Associations
  MalaCards Gene Search: ADPGK
Diseases sorted by gene-association score: monogenic diabetes (2)

-  Comparative Toxicogenomics Database (CTD)
  The following chemicals interact with this gene           more ... click here to view the complete list

-  RNA-Seq Expression Data from GTEx (53 Tissues, 570 Donors)
  Highest median expression: 43.89 RPKM in Spleen
Total median expression: 749.54 RPKM



View in GTEx track of Genome Browser    View at GTEx portal     View GTEx Body Map

+  Microarray Expression Data
  Press "+" in the title bar above to open this section.

-  mRNA Secondary Structure of 3' and 5' UTRs
 
RegionFold EnergyBasesEnergy/Base
Display As
5' UTR -28.7067-0.428 Picture PostScript Text
3' UTR -281.001047-0.268 Picture PostScript Text

The RNAfold program from the Vienna RNA Package is used to perform the secondary structure predictions and folding calculations. The estimated folding energy is in kcal/mol. The more negative the energy, the more secondary structure the RNA is likely to have.

-  Protein Domain and Structure Information
  InterPro Domains: Graphical view of domain structure
IPR007666 - ADP_PFK/GK

Pfam Domains:
PF04587 - ADP-specific Phosphofructokinase/Glucokinase conserved region

ModBase Predicted Comparative 3D Structure on Q9BRR6
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-  Orthologous Genes in Other Species
  Orthologies between human, mouse, and rat are computed by taking the best BLASTP hit, and filtering out non-syntenic hits. For more distant species reciprocal-best BLASTP hits are used. Note that the absence of an ortholog in the table below may reflect incomplete annotations in the other species rather than a true absence of the orthologous gene.
MouseRatZebrafishD. melanogasterC. elegansS. cerevisiae
Genome BrowserGenome BrowserNo orthologNo orthologNo orthologNo ortholog
Gene Details     
Gene Sorter     
MGIRGDEnsembl   
Protein SequenceProtein SequenceProtein Sequence   
AlignmentAlignmentAlignment   

-  Gene Ontology (GO) Annotations with Structured Vocabulary
  Molecular Function:
GO:0016301 kinase activity
GO:0016740 transferase activity
GO:0016773 phosphotransferase activity, alcohol group as acceptor
GO:0043843 ADP-specific glucokinase activity
GO:0046872 metal ion binding

Biological Process:
GO:0005975 carbohydrate metabolic process
GO:0006006 glucose metabolic process
GO:0006096 glycolytic process
GO:0016310 phosphorylation
GO:0061621 canonical glycolysis

Cellular Component:
GO:0005576 extracellular region
GO:0005783 endoplasmic reticulum
GO:0005789 endoplasmic reticulum membrane
GO:0016020 membrane


-  Descriptions from all associated GenBank mRNAs
  AF204270 - Homo sapiens RbBP-35 mRNA, complete cds.
AL136873 - Homo sapiens mRNA; cDNA DKFZp434B195 (from clone DKFZp434B195).
BC044589 - Homo sapiens ADP-dependent glucokinase, mRNA (cDNA clone IMAGE:5269974).
AK057651 - Homo sapiens cDNA FLJ33089 fis, clone TRACH2000497, weakly similar to Transacylases.
AK075560 - Homo sapiens cDNA PSEC0260 fis, clone NT2RP3004059.
AK055526 - Homo sapiens cDNA FLJ30964 fis, clone HEART2000035, weakly similar to Transacylases.
AK095995 - Homo sapiens cDNA FLJ38676 fis, clone IMR322000383.
AL832254 - Homo sapiens mRNA; cDNA DKFZp667C1917 (from clone DKFZp667C1917).
AK293282 - Homo sapiens cDNA FLJ55953 complete cds, highly similar to ADP-dependent glucokinase (EC 2.7.1.147).
JD130693 - Sequence 111717 from Patent EP1572962.
JD565496 - Sequence 546520 from Patent EP1572962.
JD536274 - Sequence 517298 from Patent EP1572962.
JD288636 - Sequence 269660 from Patent EP1572962.
JD154414 - Sequence 135438 from Patent EP1572962.
JD380069 - Sequence 361093 from Patent EP1572962.
AK294391 - Homo sapiens cDNA FLJ56905 complete cds, highly similar to ADP-dependent glucokinase (EC 2.7.1.147).
JD512953 - Sequence 493977 from Patent EP1572962.
JD234573 - Sequence 215597 from Patent EP1572962.
JD084753 - Sequence 65777 from Patent EP1572962.
JD510168 - Sequence 491192 from Patent EP1572962.
CR533447 - Homo sapiens full open reading frame cDNA clone RZPDo834H1116D for gene DKFZP434B195, hypothetical protein DKFZp434B195; complete cds, incl. stopcodon.
JD060980 - Sequence 42004 from Patent EP1572962.
JD037526 - Sequence 18550 from Patent EP1572962.
BC006112 - Homo sapiens ADP-dependent glucokinase, mRNA (cDNA clone MGC:12975 IMAGE:3347312), complete cds.
KJ903342 - Synthetic construct Homo sapiens clone ccsbBroadEn_12736 ADPGK gene, encodes complete protein.
KJ905562 - Synthetic construct Homo sapiens clone ccsbBroadEn_15178 ADPGK gene, encodes complete protein.
EU831454 - Synthetic construct Homo sapiens clone HAIB:100066483; DKFZo008E0617 ADP-dependent glucokinase protein (ADPGK) gene, encodes complete protein.
EU831541 - Synthetic construct Homo sapiens clone HAIB:100066570; DKFZo004E0618 ADP-dependent glucokinase protein (ADPGK) gene, encodes complete protein.
CU675251 - Synthetic construct Homo sapiens gateway clone IMAGE:100019680 5' read ADPGK mRNA.
BC018074 - Homo sapiens ADP-dependent glucokinase, mRNA (cDNA clone IMAGE:4791959), complete cds.
JD406653 - Sequence 387677 from Patent EP1572962.

-  Biochemical and Signaling Pathways
  Reactome (by CSHL, EBI, and GO)

Protein Q9BRR6 (Reactome details) participates in the following event(s):

R-HSA-5696021 ADPGK:Mg2+ phosphorylates Glc to G6P
R-HSA-70171 Glycolysis
R-HSA-70326 Glucose metabolism
R-HSA-71387 Metabolism of carbohydrates
R-HSA-1430728 Metabolism

-  Other Names for This Gene
  Alternate Gene Symbols: ADPGK_HUMAN, ENST00000456471.1, ENST00000456471.2, NM_001365229, PSEC0260, Q49AU7, Q8NBI1, Q8WZ90, Q96NF8, Q9BRR6, Q9H0A7, uc059ldf.1, uc059ldf.2
UCSC ID: ENST00000456471.3
RefSeq Accession: NM_001365225
Protein: Q9BRR6 (aka ADPGK_HUMAN)

-  Methods, Credits, and Use Restrictions
  Click here for details on how this gene model was made and data restrictions if any.