Human Gene ADPRS (ENST00000373178.5) from GENCODE V44
  Description: Homo sapiens ADP-ribosylserine hydrolase (ADPRS), mRNA. (from RefSeq NM_017825)
RefSeq Summary (NM_017825): This gene encodes a member of the ADP-ribosylglycohydrolase family. The encoded enzyme catalyzes the removal of ADP-ribose from ADP-ribosylated proteins. This enzyme localizes to the mitochondria, in addition to the nucleus and cytoplasm.[provided by RefSeq, Feb 2009].
Gencode Transcript: ENST00000373178.5
Gencode Gene: ENSG00000116863.11
Transcript (Including UTRs)
   Position: hg38 chr1:36,088,892-36,093,932 Size: 5,041 Total Exon Count: 6 Strand: +
Coding Region
   Position: hg38 chr1:36,088,905-36,093,386 Size: 4,482 Coding Exon Count: 6 

Page IndexSequence and LinksUniProtKB CommentsPrimersRNA-Seq ExpressionMicroarray Expression
RNA StructureProtein StructureOther SpeciesGO AnnotationsmRNA DescriptionsPathways
Other NamesMethods
Data last updated at UCSC: 2023-08-18 00:09:47

-  Sequence and Links to Tools and Databases
 
Genomic Sequence (chr1:36,088,892-36,093,932)mRNA (may differ from genome)Protein (363 aa)
Gene SorterGenome BrowserOther Species FASTATable SchemaAlphaFoldBioGPS
EnsemblEntrez GeneExonPrimerGencodeGeneCardsHPRD
MalacardsMGIneXtProtOMIMPubMedReactome
UniProtKBBioGrid CRISPR DB

-  Comments and Description Text from UniProtKB
  ID: ARHL2_HUMAN
DESCRIPTION: RecName: Full=Poly(ADP-ribose) glycohydrolase ARH3; EC=3.2.1.143; AltName: Full=ADP-ribosylhydrolase 3; AltName: Full=[Protein ADP-ribosylarginine] hydrolase-like protein 2;
FUNCTION: Poly(ADP-ribose) synthesized after DNA damage is only present transiently and is rapidly degraded by poly(ADP-ribose) glycohydrolase. Poly(ADP-ribose) metabolism may be required for maintenance of the normal function of neuronal cells. Generates ADP-ribose from poly-(ADP-ribose), but does not hydrolyze ADP- ribose-arginine, -cysteine, -diphthamide, or -asparagine bonds.
CATALYTIC ACTIVITY: Hydrolyzes poly(ADP-ribose) at glycosidic (1''-2') linkage of ribose-ribose bond to produce free ADP-ribose.
COFACTOR: Binds 2 magnesium ions per subunit.
ENZYME REGULATION: Activity is enhanced by magnesium.
SUBUNIT: Monomer (By similarity).
SUBCELLULAR LOCATION: Cytoplasm (By similarity). Nucleus.
TISSUE SPECIFICITY: Ubiquitous.
SIMILARITY: Belongs to the ADP-ribosylglycohydrolase family.
SEQUENCE CAUTION: Sequence=AAK14922.1; Type=Frameshift; Positions=Several;

-  Primer design for this transcript
 

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Click here to load the transcript sequence and exon structure into Primer3Plus

Exonprimer can design one pair of Sanger sequencing primers around every exon, located in non-genic sequence.
Click here to open Exonprimer with this transcript

To design primers for a non-coding sequence, zoom to a region of interest and select from the drop-down menu: View > In External Tools > Primer3


-  RNA-Seq Expression Data from GTEx (53 Tissues, 570 Donors)
  Highest median expression: 32.22 RPKM in Cells - EBV-transformed lymphocytes
Total median expression: 779.64 RPKM



View in GTEx track of Genome Browser    View at GTEx portal     View GTEx Body Map

+  Microarray Expression Data
  Press "+" in the title bar above to open this section.

-  mRNA Secondary Structure of 3' and 5' UTRs
 
RegionFold EnergyBasesEnergy/Base
Display As
3' UTR -212.30546-0.389 Picture PostScript Text

The RNAfold program from the Vienna RNA Package is used to perform the secondary structure predictions and folding calculations. The estimated folding energy is in kcal/mol. The more negative the energy, the more secondary structure the RNA is likely to have.

-  Protein Domain and Structure Information
  InterPro Domains: Graphical view of domain structure
IPR005502 - Ribosyl_crysJ1

Pfam Domains:
PF03747 - ADP-ribosylglycohydrolase

Protein Data Bank (PDB) 3-D Structure
MuPIT help
2FOZ - X-ray MuPIT 2FP0 - X-ray MuPIT 2G4K - X-ray


ModBase Predicted Comparative 3D Structure on Q9NX46
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-  Orthologous Genes in Other Species
  Orthologies between human, mouse, and rat are computed by taking the best BLASTP hit, and filtering out non-syntenic hits. For more distant species reciprocal-best BLASTP hits are used. Note that the absence of an ortholog in the table below may reflect incomplete annotations in the other species rather than a true absence of the orthologous gene.
MouseRatZebrafishD. melanogasterC. elegansS. cerevisiae
Genome BrowserGenome BrowserNo orthologNo orthologNo orthologNo ortholog
Gene Details     
Gene Sorter     
MGIRGDEnsembl   
Protein SequenceProtein SequenceProtein Sequence   
AlignmentAlignmentAlignment   

-  Gene Ontology (GO) Annotations with Structured Vocabulary
  Molecular Function:
GO:0004649 poly(ADP-ribose) glycohydrolase activity
GO:0016787 hydrolase activity
GO:0046872 metal ion binding

Biological Process:
GO:0071451 cellular response to superoxide

Cellular Component:
GO:0005634 nucleus
GO:0005654 nucleoplasm
GO:0005737 cytoplasm
GO:0005739 mitochondrion
GO:0005759 mitochondrial matrix
GO:0016604 nuclear body


-  Descriptions from all associated GenBank mRNAs
  AF212236 - Homo sapiens HPHase mRNA, complete cds.
AJ427295 - Homo sapiens mRNA for ecto-ADP-ribosylhydrolase (arh3 gene).
AK000453 - Homo sapiens cDNA FLJ20446 fis, clone KAT05231.
AK295283 - Homo sapiens cDNA FLJ51852 complete cds, highly similar to Homo sapiens ADP-ribosylhydrolase like 2 (ADPRHL2), mRNA.
BC014169 - Homo sapiens ADP-ribosylhydrolase like 2, mRNA (cDNA clone MGC:20733 IMAGE:4552006), complete cds.
AK316349 - Homo sapiens cDNA, FLJ79248 complete cds, highly similar to Homo sapiens ADP-ribosylhydrolase like 2 (ADPRHL2), mRNA.
AK223037 - Homo sapiens mRNA for ADP-ribosylhydrolase like 2 variant, clone: JTH00753.
CR457237 - Homo sapiens full open reading frame cDNA clone RZPDo834C0419D for gene ADPRHL2, ADP-ribosylhydrolase like 2; complete cds, incl. stopcodon.
KJ894094 - Synthetic construct Homo sapiens clone ccsbBroadEn_03488 ADPRHL2 gene, encodes complete protein.
JD095537 - Sequence 76561 from Patent EP1572962.
JD423186 - Sequence 404210 from Patent EP1572962.
JD181266 - Sequence 162290 from Patent EP1572962.
JD347575 - Sequence 328599 from Patent EP1572962.
JD338510 - Sequence 319534 from Patent EP1572962.
JD533413 - Sequence 514437 from Patent EP1572962.
JD464666 - Sequence 445690 from Patent EP1572962.
JD359566 - Sequence 340590 from Patent EP1572962.
JD561601 - Sequence 542625 from Patent EP1572962.
JD550601 - Sequence 531625 from Patent EP1572962.
JD563475 - Sequence 544499 from Patent EP1572962.
JD111365 - Sequence 92389 from Patent EP1572962.

-  Biochemical and Signaling Pathways
  Reactome (by CSHL, EBI, and GO)

Protein Q9NX46 (Reactome details) participates in the following event(s):

R-HSA-110362 POLB-Dependent Long Patch Base Excision Repair
R-HSA-110373 Resolution of AP sites via the multiple-nucleotide patch replacement pathway
R-HSA-73933 Resolution of Abasic Sites (AP sites)
R-HSA-73884 Base Excision Repair
R-HSA-73894 DNA Repair

-  Other Names for This Gene
  Alternate Gene Symbols: ADPRHL2, ARH3, ARHL2_HUMAN, ENST00000373178.1, ENST00000373178.2, ENST00000373178.3, ENST00000373178.4, NM_017825, Q53G94, Q6IAB8, Q9BY47, Q9NX46, uc001bzt.1, uc001bzt.2, uc001bzt.3, uc001bzt.4, uc001bzt.5
UCSC ID: ENST00000373178.5
RefSeq Accession: NM_017825
Protein: Q9NX46 (aka ARHL2_HUMAN)
CCDS: CCDS402.1

-  Methods, Credits, and Use Restrictions
  Click here for details on how this gene model was made and data restrictions if any.