Mouse Gene Tbn (G195540) Description and Page Index
  Description: Adult male hippocampus cDNA, RIKEN full-length enriched library, clone:C630015H02 product:taube nuss, full insert sequence.
Transcript (Including UTRs)
   Position: mm8 chr17:46,955,855-46,965,478 Size: 9,624 Total Exon Count: 7 Strand: -
Coding Region
   Position: mm8 chr17:46,956,558-46,965,460 Size: 8,903 Coding Exon Count: 7 

Page IndexSequence and LinksUniProtKB CommentsMicroarray ExpressionRNA StructureProtein Structure
Other SpeciesGO AnnotationsmRNA DescriptionsOther NamesMethods
Data last updated: 2006-02-27

-  Sequence and Links to Tools and Databases
 
Genomic Sequence (chr17:46,955,855-46,965,478)mRNA (may differ from genome)Protein (322 aa)
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-  Comments and Description Text from UniProtKB
  ID: TAF8_MOUSE
DESCRIPTION: RecName: Full=Transcription initiation factor TFIID subunit 8; AltName: Full=Protein taube nuss; AltName: Full=TBP-associated factor 8;
FUNCTION: Transcription factor TFIID is one of the general factors required for accurate and regulated initiation by RNA polymerase II. Mediates both basal and activator-dependent transcription. Plays a role in the differentiation of preadipocyte fibroblasts to adipocytes, however does not seem to play a role in differentiation of myoblasts. Required for the integration of TAF10 in the TAF complex (By similarity). May be important for survival of cells of the inner cell mass which constitute the pluripotent cell population of the early embryo.
SUBUNIT: TFIID is composed of TATA binding protein (TBP) and a number of TBP-associated factors (TAFs). Interacts with TBP, TAF1, TAF6, TAF10, TAF11 and TAF13. Component also of a small TAF complex (SMAT) containing TAF8, TAF10 and SUPT7L. Forms a heterodimer with TAF10. Interaction with TAF10 is mediated mainly via its histone fold domain while interaction with SUPT7L is via its C-terminal region (By similarity).
SUBCELLULAR LOCATION: Nucleus. Cytoplasm. Note=Localized in the cytoplasm and transported from the cytoplasm to the nucleus in some cells, possibly depending on the functional or developmental state of the cell.
TISSUE SPECIFICITY: Low level of expression throughout the brain with slightly higher expression in the hippocampus.
DEVELOPMENTAL STAGE: Expressed ubiquitously at very low levels throughout embryonic development. Higher levels of expression seen in inner cell mass and heart.
DISRUPTION PHENOTYPE: Death during early embryonic development. The inner cell mass cells of mutant embryos died of apoptosis.
MISCELLANEOUS: 'Taube nuss' means "empty nut' in German.
SIMILARITY: Belongs to the TAF8 family.
SIMILARITY: Contains 1 histone-fold domain.

-  Microarray Expression Data
 
Expression ratio colors:

GNF Expression Atlas 2 Data from GNF1M Mouse Chip

    
     
      
       
     
    
   
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Rinn et. al Sex Gene Expression Data on MOE430A Chip

        
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GNF Expression Atlas Data from U74b Mouse Chip

          
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Affymetrix All Exon Microarrays

           
Ratios

-  mRNA Secondary Structure of 3' and 5' UTRs
 
RegionFold EnergyBasesEnergy/Base
Display As
5' UTR -1.3018-0.072 Picture PostScript Text
3' UTR -235.06703-0.334 Picture PostScript Text

The RNAfold program from the Vienna RNA Package is used to perform the secondary structure predictions and folding calculations. The estimated folding energy is in kcal/mol. The more negative the energy, the more secondary structure the RNA is likely to have.

-  Protein Domain and Structure Information
  InterPro Domains: Graphical view of domain structure
IPR006565 - BTP
IPR009072 - Histone-fold
IPR019473 - TFIID_su8_C

Pfam Domains:
PF07524 - Bromodomain associated
PF10406 - Transcription factor TFIID complex subunit 8 C-term

ModBase Predicted Comparative 3D Structure on Q9EQH4
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-  Orthologous Genes in Other Species
  Orthologies between human, mouse, and rat are computed by taking the best BLASTP hit, and filtering out non-syntenic hits. For more distant species reciprocal-best BLASTP hits are used. Note that the absence of an ortholog in the table below may reflect incomplete annotations in the other species rather than a true absence of the orthologous gene.
HumanRatZebrafishD. melanogasterC. elegansS. cerevisiae
Genome BrowserNo orthologGenome BrowserNo orthologGenome BrowserGenome Browser
Gene Details   Gene DetailsGene Details
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GenBank Ensembl WormBaseSGD
Protein Sequence Protein Sequence Protein SequenceProtein Sequence
Alignment Alignment AlignmentAlignment

-  Gene Ontology (GO) Annotations with Structured Vocabulary
  Molecular Function:
GO:0005515 protein binding
GO:0046982 protein heterodimerization activity

Biological Process:
GO:0001833 inner cell mass cell proliferation
GO:0006351 transcription, DNA-templated
GO:0006355 regulation of transcription, DNA-templated
GO:0007275 multicellular organism development
GO:0030154 cell differentiation
GO:0045598 regulation of fat cell differentiation
GO:0045893 positive regulation of transcription, DNA-templated
GO:0051457 maintenance of protein location in nucleus

Cellular Component:
GO:0005634 nucleus
GO:0005654 nucleoplasm
GO:0005669 transcription factor TFIID complex
GO:0005737 cytoplasm
GO:0048471 perinuclear region of cytoplasm


-  Descriptions from all associated GenBank mRNAs
  AK162110 - Mus musculus in vitro fertilized eggs cDNA, RIKEN full-length enriched library, clone:7420420E18 product:taube nuss, full insert sequence.
AK045108 - Mus musculus 9.5 days embryo parthenogenote cDNA, RIKEN full-length enriched library, clone:B130033G15 product:taube nuss, full insert sequence.
AK086656 - Mus musculus 15 days embryo head cDNA, RIKEN full-length enriched library, clone:D930044C23 product:taube nuss, full insert sequence.
BC023756 - Mus musculus TAF8 RNA polymerase II, TATA box binding protein (TBP)-associated factorq, mRNA (cDNA clone IMAGE:5354624).
AF222802 - Mus musculus taube nuss mRNA, complete cds.
AK076486 - Mus musculus 0 day neonate head cDNA, RIKEN full-length enriched library, clone:4833424M19 product:taube nuss, full insert sequence.
AK161658 - Mus musculus 8 days embryo whole body cDNA, RIKEN full-length enriched library, clone:5730445M02 product:taube nuss, full insert sequence.
BC057895 - Mus musculus TAF8 RNA polymerase II, TATA box binding protein (TBP)-associated factorq, mRNA (cDNA clone MGC:68125 IMAGE:5031867), complete cds.
AK049949 - Mus musculus adult male hippocampus cDNA, RIKEN full-length enriched library, clone:C630015H02 product:taube nuss, full insert sequence.

-  Other Names for This Gene
  Alternate Gene Symbols: FLJ32821, NM_022015, TAF8
UCSC ID: AK049949
RefSeq Accession: NM_022015
Protein: Q9EQH4 (aka TAF8_MOUSE)

-  Methods, Credits, and Use Restrictions
  Click here for details on how this gene model was made and data restrictions if any.