Human Gene TAF8 (uc003orr.3) Description and Page Index
Description: Homo sapiens TAF8 RNA polymerase II, TATA box binding protein (TBP)-associated factor, 43kDa (TAF8), mRNA. RefSeq Summary (NM_138572): This gene encodes one of several TATA-binding protein (TBP)-associated factors (TAFs), which are integral subunits of the general transcription factor complex TFIID. TFIID recognizes the core promoter of many genes and nucleates the assembly of a transcription preinitiation complex containing RNA polymerase II and other initiation factors. The protein encoded by this gene contains an H4-like histone fold domain, and interacts with several subunits of TFIID including TBP and the histone-fold protein TAF10. Alternatively spliced transcript variants have been described, but their biological validity has not been determined. [provided by RefSeq, Jul 2008]. ##Evidence-Data-START## Transcript exon combination :: SRR1660807.214491.1, SRR1803614.40703.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000372977.8/ ENSP00000362068.3 RefSeq Select criteria :: based on conservation, expression ##RefSeq-Attributes-END## Transcript (Including UTRs) Position: hg19 chr6:42,018,251-42,025,516 Size: 7,266 Total Exon Count: 5 Strand: + Coding Region Position: hg19 chr6:42,018,280-42,025,287 Size: 7,008 Coding Exon Count: 5
ID:TAF8_HUMAN DESCRIPTION: RecName: Full=Transcription initiation factor TFIID subunit 8; AltName: Full=Protein taube nuss; AltName: Full=TBP-associated factor 43 kDa; AltName: Full=TBP-associated factor 8; AltName: Full=Transcription initiation factor TFIID 43 kDa subunit; Short=TAFII-43; Short=TAFII43; Short=hTAFII43; FUNCTION: Transcription factor TFIID is one of the general factors required for accurate and regulated initiation by RNA polymerase II. Mediates both basal and activator-dependent transcription. Plays a role in the differentiation of preadipocyte fibroblasts to adipocytes, however, does not seem to play a role in differentiation of myoblasts. Required for the integration of TAF10 in the TAF complex. May be important for survival of cells of the inner cell mass which constitute the pluripotent cell population of the early embryo (By similarity). SUBUNIT: TFIID is composed of TATA binding protein (TBP) and a number of TBP-associated factors (TAFs). Interacts with TBP, TAF1, TAF6, TAF10, TAF11 and TAF13. Component also of a small TAF complex (SMAT) containing TAF8, TAF10 and SUPT7L. Forms a heterodimer with TAF10. Interaction with TAF10 is mediated mainly via its histone fold domain while interaction with SUPT7L is via its C-terminal region. SUBCELLULAR LOCATION: Nucleus. Cytoplasm (By similarity). Note=Localized in the cytoplasm and transported from the cytoplasm to the nucleus in some cells, possibly depending on the functional or developmental state of the cell (By similarity). INDUCTION: Induced with triglyceride accumulation. DOMAIN: Ectopic expression of the histone fold domain acts as a dominant-negative mutant resulting in differentiation inhibition. MISCELLANEOUS: 'Taube nuss' means "empty nut' in German. SIMILARITY: Belongs to the TAF8 family. SIMILARITY: Contains 1 histone-fold domain. SEQUENCE CAUTION: Sequence=BAB71460.1; Type=Frameshift; Positions=240;
Genetic Association Studies of Complex Diseases and Disorders
Genetic Association Database (archive): TAF8 CDC HuGE Published Literature: TAF8
The RNAfold program from the Vienna RNA Package is used to perform the secondary structure predictions and folding calculations. The estimated folding energy is in kcal/mol. The more negative the energy, the more secondary structure the RNA is likely to have.
ModBase Predicted Comparative 3D Structure on Q7Z7C8
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Orthologous Genes in Other Species
Orthologies between human, mouse, and rat are computed by taking the best BLASTP hit, and filtering out non-syntenic hits. For more distant species reciprocal-best BLASTP hits are used. Note that the absence of an ortholog in the table below may reflect incomplete annotations in the other species rather than a true absence of the orthologous gene.
Biological Process: GO:0001833 inner cell mass cell proliferation GO:0006351 transcription, DNA-templated GO:0006355 regulation of transcription, DNA-templated GO:0007275 multicellular organism development GO:0030154 cell differentiation GO:0042795 snRNA transcription from RNA polymerase II promoter GO:0045598 regulation of fat cell differentiation GO:0045893 positive regulation of transcription, DNA-templated GO:0051457 maintenance of protein location in nucleus
R-HSA-6810234 General transcription factors bind SNAPc:POU2F1:ZNF143:snRNA gene R-HSA-6810238 RNA polymerase II binds initiation factors at promoter of snRNA gene (U1, U2, U4, U4atac, U5, U11, U12) R-HSA-6810235 RPAP2 binds RNA polymerase II phosphorylated at serine-7 residues of heptad repeats in the C-terminal domain R-HSA-6810233 CDK7 phosphorylates serine-5 and serine-7 of heptad repeats in C-terminal domain of RNA polymerase II at snRNA promoter R-HSA-6814885 CBCAP complex binds 7-methylguanosine cap of snRNA R-HSA-6807505 RNA polymerase II transcribes snRNA genes R-HSA-73857 RNA Polymerase II Transcription R-HSA-74160 Gene expression (Transcription)