Mol Cell Biochem 2011,
PMID: 21461609
Khodagholi, Fariba; Tusi, Solaleh Khoramian
The inflammatory reaction plays an important role in the pathogenesis of the neurodegenerative disorders. tert-butylhydroquinone (tBHQ) exhibits a wide range of pharmacological activities including anti-oxidative and anti-inflammatory action. In this study, we tried to elucidate possible effects of tBHQ on lipopolysaccharide (LPS)-induced inflammatory reaction and its underlying mechanism in neuron-like PC12 cells. tBHQ inhibited LPS-induced generation of reactive oxygen species (ROS) and elevation of intracellular calcium level. It also inhibited LPS-induced cyclooxygenase 2 (COX-2), TNF-α, nuclear factor KappaB (NF-kB), and caspase-3 expression in a dose-dependent manner while stabilizing nuclear factor-erythroid 2 p45-related factor 2. Moreover, the phosphorylations of p38, ERK1/2, and JNK were suppressed by tBHQ. These results suggest that the anti-inflammatory properties of tBHQ might result from inhibition of COX-2 and TNF-α expression, inhibition of NF-kB nuclear translocation along with suppression of MAP kinases (p38, ERK1/2, and JNK) phosphorylation in PC12 cells, so may be a useful agent for prevention of inflammatory diseases.
Diseases/Pathways annotated by Medline MESH: Calcium Signaling
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Text Mining Data
TNF-a → LPS: "
It also inhibited
LPS induced cyclooxygenase 2 (COX-2),
TNF-a , nuclear factor KappaB (NF-kB), and caspase-3 expression in a dose dependent manner while stabilizing nuclear factor-erythroid 2 p45 related factor 2
"
caspase-3 → LPS: "
It also inhibited LPS induced cyclooxygenase 2 (COX-2), TNF-a, nuclear factor KappaB (NF-kB), and caspase-3 expression in a dose dependent manner while stabilizing nuclear factor-erythroid 2 p45 related factor 2
"
nuclear factor KappaB (NF-kB) → LPS: "
It also inhibited LPS induced cyclooxygenase 2 (COX-2), TNF-a, nuclear factor KappaB (NF-kB) , and caspase-3 expression in a dose dependent manner while stabilizing nuclear factor-erythroid 2 p45 related factor 2
"
cyclooxygenase 2 (COX-2) → LPS: "
It also inhibited LPS induced cyclooxygenase 2 (COX-2) , TNF-a, nuclear factor KappaB (NF-kB), and caspase-3 expression in a dose dependent manner while stabilizing nuclear factor-erythroid 2 p45 related factor 2
"
Manually curated Databases
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