Gene interactions and pathways from curated databases and text-mining
Mol Cell Biol 1998, PMID: 9710619

A human RNA polymerase II complex containing factors that modify chromatin structure.

Cho, H; Orphanides, G; Sun, X; Yang, X J; Ogryzko, V; Lees, E; Nakatani, Y; Reinberg, D

We have isolated a human RNA polymerase II complex that contains chromatin structure remodeling activity and histone acetyltransferase activity. This complex contains the Srb proteins, the Swi-Snf complex, and the histone acetyltransferases CBP and PCAF in addition to RNA polymerase II. Notably, the general transcription factors are absent from this complex. The complex was purified by two different methods: conventional chromatography and affinity chromatography using antibodies directed against CDK8, the human homolog of the yeast Srb10 protein. Protein interaction studies demonstrate a direct interaction between RNA polymerase II and the histone acetyltransferases p300 and PCAF. Importantly, p300 interacts specifically with the nonphosphorylated, initiation-competent form of RNA polymerase II. In contrast, PCAF interacts with the elongation-competent, phosphorylated form of RNA polymerase II.

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Text Mining Data

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Manually curated Databases

  • IRef Biogrid Interaction: CDK8 — KAT2B (physical association, affinity chromatography technology)
  • IRef Biogrid Interaction: CDK8 — POLR2A (colocalization, biochemical)
  • IRef Biogrid Interaction: CDK8 — POLR2A (physical association, affinity chromatography technology)
  • IRef Biogrid Interaction: KAT2B — MED21 (physical association, affinity chromatography technology)
  • IRef Biogrid Interaction: SMARCA2 — CDK8 (colocalization, biochemical)
  • IRef Biogrid Interaction: POLR2A — SMARCB1 (colocalization, biochemical)
  • IRef Biogrid Interaction: KAT2B — POLR2A (physical association, affinity chromatography technology)
  • IRef Biogrid Interaction: GTF2F1 — POLR2A (colocalization, biochemical)
  • IRef Biogrid Interaction: EP300 — POLR2A (physical association, affinity chromatography technology)
  • IRef Biogrid Interaction: CDK8 — SMARCC2 (colocalization, biochemical)
  • IRef Biogrid Interaction: CREBBP — POLR2A (physical association, affinity chromatography technology)
  • IRef Biogrid Interaction: CREBBP — POLR2A (colocalization, biochemical)
  • IRef Biogrid Interaction: CDK8 — CREBBP (colocalization, biochemical)
  • IRef Biogrid Interaction: CDK8 — CREBBP (physical association, affinity chromatography technology)
  • IRef Biogrid Interaction: CCNC — CREBBP (colocalization, biochemical)
  • IRef Biogrid Interaction: MED21 — CREBBP (physical association, affinity chromatography technology)
  • IRef Biogrid Interaction: MED21 — POLR2A (colocalization, biochemical)
  • IRef Biogrid Interaction: MED21 — POLR2A (physical association, affinity chromatography technology)
  • IRef Biogrid Interaction: KAT2B — SMARCB1 (physical association, affinity chromatography technology)
  • IRef Biogrid Interaction: SMARCA2 — POLR2A (colocalization, biochemical)
  • IRef Biogrid Interaction: MED21 — SMARCB1 (physical association, affinity chromatography technology)
  • IRef Biogrid Interaction: KAT2B — CREBBP (physical association, affinity chromatography technology)
  • IRef Biogrid Interaction: KAT2B — CREBBP (colocalization, biochemical)
  • IRef Biogrid Interaction: CCNC — CDK8 (colocalization, biochemical)
  • IRef Biogrid Interaction: CDK8 — SMARCB1 (colocalization, biochemical)
  • IRef Biogrid Interaction: CDK8 — SMARCB1 (physical association, affinity chromatography technology)
  • IRef Biogrid Interaction: CREBBP — SMARCB1 (physical association, affinity chromatography technology)
  • IRef Biogrid Interaction: CREBBP — SMARCB1 (colocalization, biochemical)
  • IRef Biogrid Interaction: SMARCC1 — CDK8 (colocalization, biochemical)
  • IRef Biogrid Interaction: CDK8 — MED21 (colocalization, biochemical)
  • MIPS CORUM RNA polymerase II complex (RPB1, RAP74, CDK8, CYCC, SRB7, BAF190, BAF47), chromatin structure modifying: RNA polymerase II complex (RPB1, RAP74, CDK8, CYCC, SRB7, BAF190, BAF47), chromatin structure modifying complex (CCNC-CDK8-GTF2F1-MED21-POLR2A-SMARCB1-SMARCA2-SMARCA4)
  • MIPS CORUM RNA polymerase II complex (CBP, PCAF, RPB1, BAF47, CYCC, CDK8), chromatin structure modifying: RNA polymerase II complex (CBP, PCAF, RPB1, BAF47, CYCC, CDK8), chromatin structure modifying complex (CCNC-CDK8-CREBBP-KAT2B-POLR2A-SMARCB1)
  • MIPS CORUM RNA polymerase II complex, incomplete (CBP, RPBI, PCAF, BAF47), chromatin structure modifying: RNA polymerase II complex, incomplete (CBP, RPBI, PCAF, BAF47), chromatin structure modifying complex (CREBBP-KAT2B-POLR2A-SMARCB1)
  • MIPS CORUM RNA polymerase II complex, chromatin structure modifying: RNA polymerase II complex, chromatin structure modifying complex (CCNC-CDK8-DRAP1-GTF2B-GTF2E1-GTF2F1-GTF2H1-MED21-PCSK4-POLR2A-SMARCB1-SMARCC1-SMARCC2-TBP-SMARCA2-SMARCA4-SMARCD1-SMARCD2-SMARCD3)
  • MIPS CORUM RNA polymerase II complex, chromatin structure modifying: RNA polymerase II complex, chromatin structure modifying complex (ACTL6A-CCNC-CDK8-MED21-SMARCB1-SMARCC1-SMARCC2-SMARCE1-SMARCD1-SMARCD2-SMARCD3)
  • MIPS CORUM RNA polymerase II complex, chromatin structure modifying: RNA polymerase II complex, chromatin structure modifying complex (CCNC-CDK8-CREBBP-ERCC3-GTF2B-GTF2F1-GTF2H3-MED21-KAT2B-POLR2A-SMARCA2-SMARCA4-SMARCB1)
  • MIPS CORUM RNA polymerase II complex, incomplete (CDK8 complex), chromatin structure modifying: RNA polymerase II complex, incomplete (CDK8 complex), chromatin structure modifying complex (CCNC-CCNH-CDK8-GTF2F1-MED21-SMARCB1-SMARCC1-SMARCC2)
  • IRef Corum Interaction: Complex of CCNC-POLR2A-CDK8-SMARCB1-CREBBP-KAT2B (association, ion exchange chromatography)
  • IRef Corum Interaction: Complex of POLR2A-POLR2A-SMARCB1-SMARCB1-CREBBP-KAT2B-CREBBP-KAT2B (association, far western blotting)
  • IRef Corum Interaction: Complex of 27 proteins (association, far western blotting)
  • IRef Corum Interaction: Complex of SMARCA4-SMARCA2-POLR2A-CCNC-CDK8-GTF2F1-SMARCB1-MED21 (association, far western blotting)
  • IRef Corum Interaction: Complex of 20 proteins (association, affinity chromatography technology)
  • IRef Corum Interaction: Complex of 12 proteins (association, far western blotting)
  • IRef Corum Interaction: Complex of CCNC-GTF2F1-CDK8-CCNH-SMARCB1-MED21-SMARCC2-SMARCC1 (association, far western blotting)
  • IRef Hprd Interaction: Complex of 82 proteins (in vivo)
  • IRef Hprd Interaction: CREBBP — POLR2A (in vitro)
  • IRef Hprd Interaction: CREBBP — POLR2A (in vivo)
  • IRef Hprd Interaction: ACTL6A — POLR2A (in vitro)
  • IRef Hprd Interaction: ACTL6A — POLR2A (in vivo)
  • IRef Hprd Interaction: MED21 — POLR2A (in vivo)
  • IRef Hprd Interaction: CDK8 — MED21 (in vivo)
  • IRef Ophid Interaction: POLR2A — ENTHD2 (aggregation, confirmational text mining)
  • IRef Ophid Interaction: POLR2A — SMARCC1 (aggregation, confirmational text mining)
  • IRef Ophid Interaction: SMARCB1 — POLR2A (aggregation, confirmational text mining)
  • IRef Ophid Interaction: POLR2A — GTF2H1 (aggregation, confirmational text mining)
  • IRef Ophid Interaction: POLR2A — CREBBP (aggregation, confirmational text mining)
  • IRef Ophid Interaction: SMARCC2 — POLR2A (aggregation, confirmational text mining)
  • IRef Ophid Interaction: POLR2A — MED21 (aggregation, confirmational text mining)
  • IRef Ophid Interaction: SMARCA2 — POLR2A (aggregation, confirmational text mining)
  • IRef Ophid Interaction: POLR2A — SMARCE1 (aggregation, confirmational text mining)
In total, 243 gene pairs are associated to this article in curated databases