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CAV1 — EGFR

Pathways - manually collected, often from reviews:

• OpenBEL Selventa BEL large corpus: EGFR → Complex of CAV1-EGFR (directlyDecreases, CAV1/EGFR Activity) Couet et al., J Biol Chem 1997*
  Evidence: In addition, the scaffolding domains of caveolins 1 and 3 both functionally inhibit the autophosphorylation of the EGF-R kinase in vitro
• OpenBEL Selventa BEL large corpus: CAV1 → EGFR (increases, CAV1 Activity)
  Evidence: We postulated that phosphorylation could provide the stimulus for caveolae assembly. To this end, we transfected cells with mutant forms of Cav1 that could not be phosphorylated (Cav1Y14F) and tested if this altered protein reduced the number of EGF-induced caveolae. We observed that EGF-stimulated PANC-1 cells expressing the mutant Cav1Y14F protein exhibited a 90-95% reduction in caveolae number compared with cells expressing wild type Cav1.
• OpenBEL Selventa BEL large corpus: CAV1 → EGFR (increases, CAV1 Activity)
  Evidence: This is taken from supplemental table 2 from PMID 15951569 Cut-offs were 2.25 for increase and 0.5 for decrease LLID were found by converting from GI assession numbers in DAVID Spreadsheet is saved in the project folder for Cell Signaling Project # bduckworth

Protein-Protein interactions - manually collected from original source literature:

Studies that report less than 10 interactions are marked with *

• IRef Biogrid Interaction: EGFR — CAV1 (direct interaction, pull down) Couet et al., J Biol Chem 1997*
• IRef Biogrid Interaction: EGFR — CAV1 (physical association, affinity chromatography technology) Couet et al., J Biol Chem 1997*
• IRef Biogrid Interaction: EGFR — CAV1 (physical association, affinity chromatography technology) Khan et al., J Biol Chem 2006*
• IRef Biogrid Interaction: EGFR — CAV1 (physical association, affinity chromatography technology) Feldman et al., Biochim Biophys Acta 2009*
• IRef Biogrid Interaction: EGFR — CAV1 (physical association, affinity chromatography technology) Hua et al., American journal of physiology. Renal physiology 2003*
• IRef Hprd Interaction: CAV1 — EGFR (in vitro) Wu et al., Mol Cell Proteomics 2006, Couet et al., J Biol Chem 1997*
• IRef Hprd Interaction: CAV1 — EGFR (in vivo) Wu et al., Mol Cell Proteomics 2006, Couet et al., J Biol Chem 1997*
• IRef Innatedb Interaction: CAV1 — EGFR (unknown, -) Wu et al., Mol Cell Proteomics 2006
• IRef Intact Interaction: Complex of 23 proteins (association, anti bait coimmunoprecipitation) Thelemann et al., Mol Cell Proteomics 2005
• IRef Intact Interaction: Complex of 70 proteins (association, anti bait coimmunoprecipitation) Thelemann et al., Mol Cell Proteomics 2005
• IRef Intact Interaction: Complex of 20 proteins (association, anti bait coimmunoprecipitation) Wu et al., Mol Cell Proteomics 2006
• IRef Intact Interaction: Complex of CAV1-S100A9-SLC3A2-SFN-CLTA-GRB2-GAPDH-EGFR-LYN (association, anti bait coimmunoprecipitation) Wu et al., Mol Cell Proteomics 2006
• IRef Ophid Interaction: CAV1 — EGFR (aggregation, interologs mapping) Brown et al., Bioinformatics 2005

Text-mined interactions from Literome

Liu et al., Kidney Int 2005 : Depletion of caveolin-1 also significantly reduced the ouabain induced accumulation of Na/K-ATPase alpha-1 subunit, EGFR , Src, and MAPKs in clathrin-coat vesicles, as well as early and late endosomes
Kim et al., Exp Mol Pathol 2006 (Colorectal Neoplasms) : In conclusion, caveolin-1 is more expressed in cancer tissues than normal colon and related with Akt-1, not with EGFR expression in colorectal cancer tissues, which suggests that signaling for caveolin-1 affects Akt-1 activation, but this reaction is not initiated by EGFR stimulation in colon cancer
Zhang et al., Cell Signal 2007 : Caveolin-1 phosphorylation is required for stretch induced EGFR and Akt activation in mesangial cells
Gu et al., Zhonghua Zhong Liu Za Zhi 2007 (Carcinoma, Squamous Cell...) : EGFR was found to colocalize with caveolin-1 in transfected cells by confocal laser scanning microscopy and Western blotting results showed that overexpression of caveolin-1 reduced the phosphorylation of EGFR and Erkl/2
Wang et al., Cancer Res 2007 (Carcinoma, Squamous Cell) : Suppression of epidermal growth factor receptor signaling by protein kinase C-alpha activation requires CD82, caveolin-1 , and ganglioside
Tencer et al., Anticancer Res 2008 : Our findings indicate that up-regulation of caveolin-1 by rosiglitazone requires superoxide formation and the activation of Src, EGFR , and the Mek1-Erk1/2 and p38 MAP kinase pathways
Dittmann et al., Molecular cancer 2008 : Inhibition of EGFR by the antibody Erbitux resulted in a strong accumulation of caveolin/EGFR complexes within the cytoplasm, which could not be further increased by irradiation
Thomas et al., Breast Cancer Res Treat 2010 (Breast Neoplasms) : Recombinant expression of caveolin-1 in TAM-R cells did not affect EGFR/ERK activity, potentially due to mislocalisation of caveolin-1 through hyperactivation of the mTOR pathway or altered caveolin-1 phosphorylation
Agelaki et al., Cancer Biol Ther 2009 (Adenocarcinoma...) : Caveolin-1 regulates EGFR signaling in MCF-7 breast cancer cells and enhances gefitinib induced tumor cell inhibition ... In this report we investigated the role of caveolin-1 overexpression on EGFR signaling in MCF-7 breast cancer cell line
Chen et al., Mol Cell Biol 2010 : Integrin alpha1beta1 regulates epidermal growth factor receptor activation by controlling peroxisome proliferator activated receptor gamma dependent caveolin-1 expression ... Thus, integrin alpha1beta1 negatively regulates EGFR activation by positively controlling Cav-1 levels, and the ERK/PPARgamma axis plays a key role in regulating integrin alpha1beta1 dependent Cav-1 expression and consequent EGFR mediated ROS production
Zuo et al., Breast Cancer Res 2010 (Breast Neoplasms...) : To define the potential inter-mediate steps between mPRalpha and PI3K, we demonstrated that mPRalpha, caveolin-1 (Cav-1), and epidermal growth factor receptor (EGFR) are colocalized in the membrane of caveolar vesicle and the P4-repressed EMT in MB468 cells can be blocked by EGFR inhibitor ( AG1478 ) and PI3K inhibitor ( wortmannin )
Zhu et al., PloS one 2010 : Furthermore, we demonstrated that the expression of Cav-1 protected cells against DNA damage through modulating the activities of both the homologous recombination ( HR ) and non-homologous end joining ( NHEJ ) repair systems, as evidenced by the inhibitory effects of the Cav-1 targeted siRNA on cell survival, HR frequency, phosphorylation of DNA dependent protein kinase (DNA-PK), and nuclear translocation of epidermal growth factor receptor (EGFR) following DNA damage, and by the stimulatory effect of the forced expression of Cav-1 on NHEJ frequency
Takaguri et al., J Mol Cell Cardiol 2011 (Hypertrophy...) : Caveolin-1 negatively regulates a metalloprotease dependent epidermal growth factor receptor transactivation by angiotensin II
Schmidt-Glenewinkel et al., FEBS Lett 2012 : We applied flow cytometry, confocal microscopy and automated image quantification to define the role of Caveolin1 (Cav1) in epidermal growth factor (EGF) receptor ( i ) internalization and ( ii ) endosomal trafficking