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APOB — PLD1
Text-mined interactions from Literome
Gómez-Muñoz et al., Arterioscler Thromb Vasc Biol 2000
(Arteriosclerosis) :
Stimulation of
phospholipase D activity by oxidized
LDL in mouse peritoneal macrophages ... Pretreatment of macrophages with the protein kinase C inhibitor Ro-32-0432 or downregulation of protein kinase C activity by prolonged incubation with 100 nmol/L 4beta-phorbol 12-myristate 13-acetate did not alter the stimulatory
effect of oxidized
LDL on
PLD activation ... However, oxidized
LDL stimulated tyrosine phosphorylation of several macrophage proteins, and preincubation of the macrophages with genistein, a tyrosine kinase inhibitor,
blocked the activation of
PLD by oxidized LDL ... In addition, pretreatment with orthovanadate, which inhibits tyrosine phosphatases, enhanced basal and oxidized
LDL stimulated
PLD activity ... Pretreatment of macrophages with pertussis toxin decreased the stimulatory effect of oxidized LDL, indicating that GTP binding proteins may also be involved in the
activation of
PLD by oxidized
LDL
Natarajan et al., J Lipid Res 1995
(Arteriosclerosis) :
To test this hypothesis,
activation of
PLD by
OX-LDL was investigated in [ 3H ] myristic acid- or [ 32P ] orthophosphate labeled rabbit femoral artery smooth muscle cells ( RFASMC ) in the presence of 0.5 % ethanol or 0.05 % butanol ... To determine the mechanism ( s ) of
OX-LDL induced
PLD activation, the role of protein kinase C ( PKC ) and Ca2+ was investigated ... Pretreatment of [ 32P ] orthophosphate labeled RFASMC with known inhibitors of PKC such as staurosporine, calphostin-C, or H-7, had no effect on
OX-LDL induced
PLD activation ... In addition, exposure of RFASMC to sodium orthovanadate, an inhibitor of phosphatases, enhanced the
OX-LDL mediated
PLD activation ... The
activation of
PLD by
OX-LDL generates second-messengers like PA and/or LPA which modulate mitogenesis