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BRCA1 — RNF8

Pathways - manually collected, often from reviews:

• OpenBEL Selventa BEL large corpus: BRCA1 → Complex of MDC1-RNF8 (increases)
  Evidence: Recent evidence suggests that RNF8 binds to MDC1 and ubiquitylates phosphorylated H2AX. These events facilitate the recruitment of factors (such as BRCA1 and 53BP1) that are required for DNA repair and checkpoint signalling, probably through a ubiquitin-ligase cascade98?100,102 (FIG. 2). (primary sources used as in statements)
• OpenBEL Selventa BEL large corpus: BRCA1 → RNF8 (increases)
  Evidence: Both UBC13 and RNF8 are required for localization of BRCA1 and Abraxas to sites of damage92,102,115?117.
• NCI Pathway Database ATM pathway: MRN complex/ATM/MDC1/H2AX/RNF8/53BP1/BRCA1 complex (MRE11A-NBN-RAD50-ATM-H2AFX-MDC1-RNF8-TP53BP1-BRCA1) → MRN complex/ATM/MDC1/H2AX/RNF8/53BP1/BRCA1/ABRA1 complex (MRE11A-NBN-RAD50-ATM-H2AFX-MDC1-RNF8-TP53BP1-BRCA1-FAM175A) (modification, collaborate)
  Wang et al., Science 2007
  Evidence: mutant phenotype, physical interaction
• NCI Pathway Database ATM pathway: MRN complex/ATM/MDC1/H2AX/RNF8/53BP1/BRCA1 complex (MRE11A-NBN-RAD50-ATM-H2AFX-MDC1-RNF8-TP53BP1-BRCA1) → ABRA1 (FAM175A) (modification, collaborate) Wang et al., Science 2007
  Evidence: mutant phenotype, physical interaction
• NCI Pathway Database ATM pathway: MRN complex/ATM/MDC1/H2AX/RNF8/53BP1/BRCA1/ABRA1 complex (MRE11A-NBN-RAD50-ATM-H2AFX-MDC1-RNF8-TP53BP1-BRCA1-FAM175A) → ABRA1 (FAM175A) (modification, collaborate) Wang et al., Science 2007
  Evidence: mutant phenotype, physical interaction
• NCI Pathway Database ATM pathway: MRN complex/ATM/MDC1/H2AX/RNF8/53BP1/BRCA1 complex (MRE11A-NBN-RAD50-ATM-H2AFX-MDC1-RNF8-TP53BP1-BRCA1) → MRN complex/ATM/MDC1/H2AX/RNF8/53BP1 complex (MRE11A-NBN-RAD50-ATM-H2AFX-MDC1-RNF8-TP53BP1) (modification, collaborate)
  Cortez et al., Science 1999, Li et al., Nature 2000, Xu et al., Mol Cell Biol 2001, Stewart et al., Nature 2003, Lou et al., J Biol Chem 2003, Mailand et al., Cell 2007, Huen et al., Cell 2007, Kolas et al., Science 2007
  Evidence: mutant phenotype, assay, physical interaction
• NCI Pathway Database ATM pathway: MRN complex/ATM/MDC1/H2AX/RNF8/53BP1/BRCA1 complex (MRE11A-NBN-RAD50-ATM-H2AFX-MDC1-RNF8-TP53BP1-BRCA1) → CtBP/CtIP complex (RBBP8) (modification, collaborate) Cortez et al., Science 1999, Li et al., Nature 2000, Xu et al., Mol Cell Biol 2001, Stewart et al., Nature 2003, Lou et al., J Biol Chem 2003, Mailand et al., Cell 2007, Huen et al., Cell 2007, Kolas et al., Science 2007
  Evidence: mutant phenotype, assay, physical interaction
• NCI Pathway Database ATM pathway: MRN complex/ATM/MDC1/H2AX/RNF8/53BP1/BRCA1 complex (MRE11A-NBN-RAD50-ATM-H2AFX-MDC1-RNF8-TP53BP1-BRCA1) → BRCA1/CtBP/CtIP complex (BRCA1-CTBP1-RBBP8) (modification, collaborate)
  Cortez et al., Science 1999, Li et al., Nature 2000, Xu et al., Mol Cell Biol 2001, Stewart et al., Nature 2003, Lou et al., J Biol Chem 2003, Mailand et al., Cell 2007, Huen et al., Cell 2007, Kolas et al., Science 2007
  Evidence: mutant phenotype, assay, physical interaction
• NCI Pathway Database ATM pathway: MRN complex/ATM/MDC1/H2AX/RNF8/53BP1 complex (MRE11A-NBN-RAD50-ATM-H2AFX-MDC1-RNF8-TP53BP1) → BRCA1/CtBP/CtIP complex (BRCA1-CTBP1-RBBP8) (modification, collaborate)
  Cortez et al., Science 1999, Li et al., Nature 2000, Xu et al., Mol Cell Biol 2001, Stewart et al., Nature 2003, Lou et al., J Biol Chem 2003, Mailand et al., Cell 2007, Huen et al., Cell 2007, Kolas et al., Science 2007
  Evidence: mutant phenotype, assay, physical interaction
• NCI Pathway Database ATM pathway: MRN complex/ATM/MDC1/H2AX/RNF8/53BP1/BRCA1/ABRA1/RAP80 complex (MRE11A-NBN-RAD50-ATM-H2AFX-MDC1-RNF8-TP53BP1-BRCA1-FAM175A-UIMC1) → RAP80 (UIMC1) (modification, collaborate) Wang et al., Science 2007, Mailand et al., Cell 2007, Huen et al., Cell 2007, Kolas et al., Science 2007
  Evidence: mutant phenotype, physical interaction
• NCI Pathway Database ATM pathway: MRN complex/ATM/MDC1/H2AX/RNF8/53BP1/BRCA1/ABRA1/RAP80 complex (MRE11A-NBN-RAD50-ATM-H2AFX-MDC1-RNF8-TP53BP1-BRCA1-FAM175A-UIMC1) → MRN complex/ATM/MDC1/H2AX/RNF8/53BP1/BRCA1/ABRA1 complex (MRE11A-NBN-RAD50-ATM-H2AFX-MDC1-RNF8-TP53BP1-BRCA1-FAM175A) (modification, collaborate)
  Wang et al., Science 2007, Mailand et al., Cell 2007, Huen et al., Cell 2007, Kolas et al., Science 2007
  Evidence: mutant phenotype, physical interaction
• NCI Pathway Database ATM pathway: RAP80 (UIMC1) → MRN complex/ATM/MDC1/H2AX/RNF8/53BP1/BRCA1/ABRA1 complex (MRE11A-NBN-RAD50-ATM-H2AFX-MDC1-RNF8-TP53BP1-BRCA1-FAM175A) (modification, collaborate)
  Wang et al., Science 2007, Mailand et al., Cell 2007, Huen et al., Cell 2007, Kolas et al., Science 2007
  Evidence: mutant phenotype, physical interaction
• NCI Pathway Database ATM pathway: MRN complex/ATM/MDC1/H2AX/RNF8/53BP1/BRCA1 complex (MRE11A-NBN-RAD50-ATM-H2AFX-MDC1-RNF8-TP53BP1-BRCA1) → SMC1 (SMC1A) (modification, activates) Kim et al., Genes Dev 2002, Yazdi et al., Genes Dev 2002, Kitagawa et al., Genes Dev 2004
  Evidence: mutant phenotype, assay, physical interaction
• NCI Pathway Database ATM pathway: MRN complex/ATM/MDC1/H2AX/RNF8/53BP1/BRCA1 complex (MRE11A-NBN-RAD50-ATM-H2AFX-MDC1-RNF8-TP53BP1-BRCA1) → SMC3 (SMC3) (modification, activates) Luo et al., J Biol Chem 2008
  Evidence: mutant phenotype, assay, physical interaction
• NCI Pathway Database ATM pathway: MRN complex/ATM/MDC1/H2AX/RNF8/53BP1/BRCA1 complex (MRE11A-NBN-RAD50-ATM-H2AFX-MDC1-RNF8-TP53BP1-BRCA1) → MRN complex/ATM/MDC1/H2AX/RNF8/53BP1 complex (MRE11A-NBN-RAD50-ATM-H2AFX-MDC1-RNF8-TP53BP1) (modification, collaborate)
  Li et al., Nature 2000, Xu et al., Cancer Res 2002, Stewart et al., Nature 2003, Mailand et al., Cell 2007, Huen et al., Cell 2007, Kolas et al., Science 2007
  Evidence: mutant phenotype, assay, physical interaction
• NCI Pathway Database ATM pathway: MRN complex/ATM/MDC1/H2AX/RNF8/53BP1/BRCA1 complex (MRE11A-NBN-RAD50-ATM-H2AFX-MDC1-RNF8-TP53BP1-BRCA1) → CtBP/CtIP complex (RBBP8) (modification, collaborate) Li et al., Nature 2000, Xu et al., Cancer Res 2002, Stewart et al., Nature 2003, Mailand et al., Cell 2007, Huen et al., Cell 2007, Kolas et al., Science 2007
  Evidence: mutant phenotype, assay, physical interaction
• NCI Pathway Database ATM pathway: MRN complex/ATM/MDC1/H2AX/RNF8/53BP1/BRCA1 complex (MRE11A-NBN-RAD50-ATM-H2AFX-MDC1-RNF8-TP53BP1-BRCA1) → BRCA1/CtBP/CtIP complex (BRCA1-CTBP1-RBBP8) (modification, collaborate)
  Li et al., Nature 2000, Xu et al., Cancer Res 2002, Stewart et al., Nature 2003, Mailand et al., Cell 2007, Huen et al., Cell 2007, Kolas et al., Science 2007
  Evidence: mutant phenotype, assay, physical interaction
• NCI Pathway Database ATM pathway: MRN complex/ATM/MDC1/H2AX/RNF8/53BP1 complex (MRE11A-NBN-RAD50-ATM-H2AFX-MDC1-RNF8-TP53BP1) → BRCA1/CtBP/CtIP complex (BRCA1-CTBP1-RBBP8) (modification, collaborate)
  Li et al., Nature 2000, Xu et al., Cancer Res 2002, Stewart et al., Nature 2003, Mailand et al., Cell 2007, Huen et al., Cell 2007, Kolas et al., Science 2007
  Evidence: mutant phenotype, assay, physical interaction

Protein-Protein interactions - manually collected from original source literature:

Studies that report less than 10 interactions are marked with *

• IRef Intact Interaction: BRCA1 — RNF8 (colocalization, fluorescence microscopy) Huen et al., Cell 2007

Text-mined interactions from Literome

Kolas et al., Science 2007 : Here, we reveal that the ubiquitin ligase RNF8 mediates ubiquitin conjugation and 53BP1 and BRCA1 focal accumulation at sites of DNA lesions
Guerra-Rebollo et al., Exp Cell Res 2012 : RNF8 interacted through its FHA domain with the ribosomal protein RPSA, and knockdown of RPSA caused a depletion of nucleolar RNF8 and BRCA1 , suggesting that the interaction of RNF8 with RPSA is critical for the nucleolar localization of these DDR factors