Gene interactions and pathways from curated databases and text-mining

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Text-mined interactions from Literome

Band et al., J Biol Chem 1997 : Phosphatidylinositol 3'-kinase and p70s6k are required for insulin but not bisperoxovanadium 1,10-phenanthroline ( bpV ( phen ) ) inhibition of insulin-like growth factor binding protein gene expression
Cross et al., Nature 1995 : GSK3 is inhibited by serine phosphorylation in response to insulin or growth factors and in vitro by either MAP kinase activated protein ( MAPKAP ) kinase-1 ( also known as p90rsk ) or p70 ribosomal S6 kinase (p70S6k) ... Here we show, however, that agents which prevent the activation of both MAPKAP kinase-1 and p70S6k by insulin in vivo do not block the phosphorylation and inhibition of GSK3
Grzelkowska et al., J Endocrinol 1999 (Diabetes Mellitus, Experimental...) : Neither basal nor insulin stimulated p70 ( S6K ) activity, a signalling element lying downstream of mTOR, were modified by STZ-diabetes
Morito et al., Biol Pharm Bull 2005 : In the cells, insulin induced the phosphorylation of extracellular signal regulated kinases ( Erks ), Akt at Ser 473 and ribosomal p70 S6 protein kinase (p70 S6K) at Thr 389, and the insulin induced 2-deoxy-D- [ 1-3H ] glucose uptake was inhibited by pre-treatment with wortmannin, an inhibitor of phosphatidylinositol 3-kinase (PI3K), or ML-9, an Akt inhibitor
Fox et al., Am J Physiol 1998 : Previous studies indicated that amino acids may activate the protein kinase activity of the target of rapamycin ( TOR ) and thereby augment and/or mimic the effects of insulin on protein synthesis, p70 ( S6k ) phosphorylation, and multicellular clustering in adipocytes
Hurel et al., Biochem J 1996 : Activation by insulin of the protein kinases p70s6K , p90s6K and extracellular signal regulated kinase 2 ( ERK2 ) is observed, but is significantly slower than the activation of GS
Maeurer et al., Cell Signal 2009 : Increased intracellular PAM concentrations facilitated S1P- and insulin induced mTOR activation as well as p70S6K and 4EBP1 phosphorylation while genetic deletion of PAM decreased S1P- and insulin induced mTOR activation
Lin et al., J Biol Chem 1995 : Rapamycin abolished the effects of insulin on increasing phosphorylation of ribosomal protein S6 and on activating p70S6K
Obata et al., Circ Res 1996 : In this study, we observed that > or = 1 nmol/L insulin stimulated tyrosine phosphorylation of insulin receptor substrate-1 (IRS-1) and activated IRS-1 dependent phosphatidylinositol 3'-kinase ( PI 3'-kinase ) and p70 S6 kinase (p70S6K) but not MAP kinase ( extracellular signal regulated kinase 2 ) and p90 S6 kinase ( p90RSK )
Weng et al., J Biol Chem 1998 : Activation of p70 by insulin was accompanied by a coordinate increase in phosphorylation at all sites examined, together with a slowing in mobility on SDS-PAGE of a portion of p70 polypeptides
Whitehead et al., Biochem J 2000 : Consistent with this, contraction inhibited insulin stimulated p70 ( S6K ) activation ... Thus, in contracting skeletal muscle, insulin stimulates glucose uptake and activates PKB, but not p70 ( S6K ), by a PI 3-kinase dependent mechanism that is independent of changes in IRS-1- and IRS-2 associated PI 3-kinase activity
Shah et al., Biochem J 2002 : In the present study, we have shown that 4-amino-5- ( 4-methylphenyl ) -7- ( t-butyl ) pyrazolo [ 3,4-d ] pyrimidine ( PP1 ), a selective inhibitor of the Src family of non-receptor tyrosine kinases, interferes with the activation of 70 and 85 kDa S6K gene products ( p70S6K1 and p85S6K1 ) by insulin , insulin-like growth factor 1, sodium orthovanadate and activated alleles of phosphoinositide 3-kinase and H-Ras
Grzelkowska-Kowalczyk et al., Cell Biol Int 2010 : Treatment with IFN-gamma prevents insulin dependent PKB, p70S6k phosphorylation and protein synthesis in mouse C2C12 myogenic cells
Lin et al., J Biol Chem 1994 : Rapamycin blocked activation of p70S6K by insulin , but it did not attenuate the effect ( 2-fold ) of insulin on increasing the glycogen synthase activity ratio ( +/-glucose-6-P )
Shen et al., Pediatr Res 2005 : Insulin increased PKB/Akt phosphorylation and p70S6K phosphorylation to a similar extent as in fed animals
Miyake et al., Cell Struct Funct 1991 : Fibroblast growth factor and insulin did not induce p70
Liu et al., Am J Physiol Endocrinol Metab 2004 : To assess the physiological relevance of these findings to normal human physiology, we compared the effects of amino acids and insulin on whole body glucose disposal, p70 ( S6K ), and GSK-3 phosphorylation, and on the activity of GS in vivo in skeletal muscle of 24 healthy human volunteers
Wu et al., Endocrinology 2004 : Insulin enhanced the phosphorylation of Akt ( P < 0.04 ), 4E-BP1 ( P < 0.002 ), and p70 ( S6K ) ( P < 0.0001 ) in ADX, but not in sham rats ... Dexamethasone restored the levels of 4E-BP1 and p70 ( S6K ) phosphorylation and abrogated the insulin stimulated Akt, 4E-BP1, and p70 ( S6K ) phosphorylation
Varma et al., Biochim Biophys Acta 2007 : Therefore, the effects of insulin and rapamycin ( an inhibitor of mTOR ) on the phosphorylation of mTOR ( Ser 2448 ) and p70 ( S6K ) ( Thr 389 ) as well as on cell proliferation in parental HepG2 cells and HepG2 cells overexpressing constitutively active Akt/PKB ( HepG2-CA-Akt/PKB ) were studied ... Insulin increased the levels of phosphorylated mTOR and p70 ( S6K ) in both the cell lines
Xue et al., J Biol Chem 2007 (Diabetes Mellitus, Experimental...) : Insulin stimulated phosphorylation of IR, IRS proteins, Akt/protein kinase B, glycogen synthase kinase 3beta, and p70 ( S6K ) was impaired in DHet mouse muscle and liver and was differentially improved by PTP1B deficiency
Demeulder et al., Biochim Biophys Acta 2013 (Myocardial Ischemia) : Ischemia decreased insulin induced mTOR-p70S6K phosphorylation in WT and AMPKa2 KO mice to a similar extent ... Its deletion increased the insulin induced p70S6K stimulation
Biswas et al., Molecular vision 2009 : However, imatinib exposure did not affect insulin induced insulin receptor substrate (IRS) associated PI 3-kinase activity and the downstream phosphorylation of Akt, GSK-3beta, and p70S6kinase
Sharma et al., Mol Cell Biol 1997 : In both cell types, overexpression of either the PTB or the SAIN protein caused a significant decrease in insulin induced tyrosine phosphorylation of IRS-1 and Shc proteins, IRS-1 associated phosphatidylinositol 3-kinase (PI 3-K) enzymatic activity, p70s6k activation, and p44 and p42 mitogen activated protein kinase ( MAPK ) phosphorylation ... p70s6k is a downstream target of PI 3-K, and insulin stimulated p70s6k was inhibited by PTB or SAIN expression ... Thus, interference with the IRS-1-IR interaction inhibits insulin stimulated IRS-1 and Shc phosphorylation, PI 3-K enzymatic activity, p70s6k activation, MAPK phosphorylation and cell cycle progression
Greenhaff et al., Am J Physiol Endocrinol Metab 2008 : The phosphorylation of PKB Ser ( 473 ) and p70 ( S6k ) Thr ( 389 ) increased concomitantly with insulin , but whereas raising insulin to 30 mU/l increased the phosphorylation of mTOR Ser ( 2448 ), 4E-BP1 Thr ( 37/46 ), or GSK3beta Ser ( 9 ) and decreased that of eEF2 Thr ( 56 ), higher insulin doses to 72 and 167 mU/l did not augment these latter responses
Terruzzi et al., Acta Diabetol 2005 : INS stimulated p70S6k and 4E-BP1 phosphorylation transiently in HUVEC and persistently in HVSMC ... AA and INS+AA stimulated p70S6k and 4E-BP1 phosphorylation persistently in HUVEC and HVSMC
Long et al., Am J Physiol Endocrinol Metab 2003 : Insulin significantly enhanced the phosphorylation of Akt ( P < 0.03 ), 4E-BP1 ( P = 0.003 ), and p70 ( S6K ) ( P < 0.002 ) in ADX but not in Sham rats ... Glucocorticoid replacement blunted the effect of insulin on Akt, 4E-BP1, and p70 ( S6K ) phosphorylation and protein synthesis
Noda et al., J Med Invest 2000 : Overexpression of wild-type Akt1 promoted insulin stimulated p70S6 kinase (p70S6K) activity and affected GSK3 beta regulation, but did not promote insulin stimulated GLUT4 translocation or glucose transport in L6 myotubes ... We found that overexpression of WT Akt1 promoted insulin stimulated p70S6 kinase (p70S6K) activity and increased the basal activity of GSK3 beta, but did not promote insulin stimulated GLUT4 translocation or glucose uptake
Koketsu et al., Am J Physiol Endocrinol Metab 2008 (Glucose Intolerance...) : Insulin induced activation of p70S6 kinase ( p70 ( S6k ) ) was significantly suppressed in the livers of raptor-DeltaC ( T ) overexpressing mice
Gagnon et al., J Cell Physiol 2001 : Insulin stimulated p70 S6 kinase activity, and its inhibition by rapamycin, were comparable in preadipocytes at day 0 vs. day 4 post-differentiation
González et al., Regul Pept 2005 : GLP-1 and both exendins activated, like insulin, PI3K/PKB and p42/44 MAPK enzymes, but p70s6k was activated only by GLP-1 and insulin
Fox et al., Diabetes 2006 (Diabetes Mellitus, Experimental...) : Glycosphingolipid inhibitors augmented insulin stimulated p70 S6kinase activity in the presence of inhibitory concentrations of high glucose or glucosamine
Sadiq et al., BMC molecular biology 2007 : Incubation of rapamycin ( inhibitor of mTOR ) inhibited amino acid or insulin dependent p70 S6 kinase phosphorylation, blocked ( P < 0.05 ) the inhibitory effects of 1.0 x PC AA on protein degradation, but did not alter the inhibitory effects of insulin or leucine
Ramírez-Rangel et al., Mol Cell Biol 2011 : GRp58/ERp57 knockdown reduces mTORC1 levels and phosphorylation of 4E-BP1 and p70 ( S6K ) in response to insulin
García-San Frutos et al., Endocrinology 2007 (Body Weight...) : Hypothalamic insulin stimulated insulin receptor, GSK3, AKT, and p70S6K phosphorylation decreased with aging
El-Chaâr et al., International journal of obesity and related metabolic disorders : journal of the International Association for the Study of Obesity 2004 : We measured the effect of rapamycin, a specific inhibitor of mTOR, on insulin induced 3T3-L1 adipogenesis and on insulin stimulated p70 S6K activation ... In contrast, rapamycin completely inhibited insulin stimulated p70 S6K activation, assessed by phosphorylation of p70 S6K and its substrate, S6
Longnus et al., Diabetologia 2005 : Insulin induced phosphorylation of glycogen synthase kinase 3 (GSK3) beta, p70 S6 kinase (p70S6K) ( Thr389 ) and IRS1 ( Ser636/639 ) were significantly increased following AMPK activation
Moule et al., Biochem J 1995 : Rapamycin also failed to have any appreciable effect on stimulation of these parameters by insulin, although it did block the effect of insulin on p70S6K
Bartolomé et al., Endocrinology 2010 : More importantly, upregulation of p70S6K signaling impaired insulin stimulated phosphorylation of Akt Ser ( 473 ) and p70S6K in IR ( +/+ ) and Rec B but not in Rec A cell lines
Musnier et al., Cell Mol Life Sci 2009 : At both developmental stages, insulin induced p70S6K regulation was consistent with reported data
Mendez et al., Mol Cell Biol 1997 : Ectopic expression of constitutively activated PKC zeta eliminates the requirement of IRS-1 for general protein synthesis but not for insulin stimulated activation of 70-kDa S6 kinase ( p70S6K ), synthesis of growth regulated proteins ( e.g., c-Myc ), or mitogenesis
Pellegatta et al., J Cardiovasc Pharmacol 2006 : In this work we have investigated the intracellular pathways involved in insulin induced and amino acid induced p70/S6-K activations in human endothelial cells ... Insulin and amino acids phosphorylate p70/S6-K by mTor dependent and phosphotidylinositol 3-kinase dependent mechanisms, whereas the mitogen activated protein kinase pathway is involved only when p70/S6-K is activated by insulin
Lee et al., Carcinogenesis 2009 (Carcinoma, Hepatocellular...) : Overexpression of Pin1 resulted in enhanced p70S6K phosphorylation induced by insulin in SK-HEP-1 cells ... In contrast, Pin1 ( -/- ) mouse embryonic fibroblasts ( MEFs ) exhibited significantly decreased insulin induced p70S6K phosphorylation compared with Pin1 ( +/+ ) MEFs
Onuma et al., Biochim Biophys Acta 1998 : Neither PD98059, a specific inhibitor of MAP kinase, nor rapamycin, a potent inhibitor of insulin dependent stimulation of p70 ribosomal protein S6 kinase (p70S6K) , had inhibitory effect on that of PDE kinase ... These results are consistent with the view that ( i ) insulin activated PDE kinase as well as MAP kinase and p70S6K are localized downstream of phosphatidylinositol 3-kinase, ( ii) PDE kinase is distinct from either MAP kinase or p70S6K and ( iii ) PDE kinase does not exist downstream of either MAP kinase or p70S6K
Staubs et al., J Biol Chem 1998 : Furthermore, insulin activation of Akt and p70 ( s6kinase ), kinases downstream of PI3K, was unaffected by PDGF pretreatment
Chung et al., Cell Signal 2000 : In serum starved CK expressor cells, insulin and IGF-I stimulated DNA synthesis, p70 S6 kinase (p70 S6K) activity, phosphatidylinositol 3-kinase (PI3K) activity, and activating phosphorylation of p42/p44 mitogen activated protein kinases ( MAPK ) to greater extents than in the corresponding vector control cells
Yenush et al., J Biol Chem 1996 : In 32D cells the IH1 ( PH ) was essential for insulin stimulated tyrosine phosphorylation of IRS-1 and insulin stimulated phosphatidylinositol 3-kinase activity and p70 ( s6k ) phosphorylation
Peak et al., Diabetologia 1998 : Both insulin and EGF activated extracellular regulated kinase 2 (ERK-2), p70rsk and protein kinase B (PKB) and inactivated glycogen synthase kinase-3 ( GSK-3 )
Miakotina et al., Respir Res 2002 : In contrast, insulin increased p70 S6 kinase Thr389 phosphorylation within 15 minutes
Long et al., Am J Physiol Endocrinol Metab 2000 (Body Weight) : We examined whether phosphorylation of PHAS-I and p70 ( S6k ) was increased by feeding and determined the separate effects of insulin and amino acids on PHAS-I and p70 ( S6k ) phosphorylation in rat skeletal muscle in vivo
Kim et al., Endocrinology 2011 (Hyperinsulinism...) : More importantly, acute insulin stimulation after chronic insulin treatment resulted in blunted phosphorylation of Akt, p70S6K , and glycogen synthase kinase-3ß
Hiratani et al., Biochem Biophys Res Commun 2005 : Interestingly, anisomycin induced p70 ( S6K ) phosphorylation was reduced by SP600125, while insulin induced p70 ( S6K ) phosphorylation was not
von Manteuffel et al., Proc Natl Acad Sci U S A 1996 : More striking, insulin , which does not induce p42mapk activation in human 293 cells or Swiss mouse 3T3 cells, induces 4E-BP1 phosphorylation and p70s6k activation in both cell types ... The insulin effect on 4E-BP1 phosphorylation and p70s6k activation in both cell types is blocked by SQ20006, wortmannin, and rapamycin
Dardevet et al., Endocrinology 1996 : Although p70S6K activation by insulin and IGF-I may be mediated by PI3 kinase in epitrochlearis muscle, the specific inhibition of this kinase by rapamycin caused only partial ( 25 % ) inhibition of the stimulation of protein synthesis by these two hormones
Kanazawa et al., J Biol Chem 2004 : In isolated rat hepatocytes, both regulatory amino acids ( RegAA ) and insulin coordinately activated p70 ( S6k ) phosphorylation, which was completely blocked by rapamycin, an mTOR inhibitor
Sanchez Canedo et al., Am J Physiol Endocrinol Metab 2010 : In wild-type hearts, both leucine and insulin increased p70 ( S6K ) activity whereas, in contrast to insulin, leucine was unable to activate PKB/Akt ... Moreover, the introduction in PDK1 of the L155E mutation, which is known to preserve the insulin induced and PKB/Akt dependent phosphorylation of mTOR/p70 ( S6K ), suppressed all leucine effects, including phosphorylation of mTOR, PRAS40, and p70 ( S6K )
Markuns et al., Endocrinology 1999 (Diabetes Mellitus, Experimental) : In contrast, there was a decrease in insulin stimulated p70S6k activity with no change in protein expression in the diabetic rats
Scott et al., FEBS Lett 1997 : Insulin also activated p70S6K and the Erk-1 and Erk-2 isoforms of mitogen activated protein kinase ( MAP kinase )
Wu et al., J Biol Chem 2004 : Insulin treatment induced p70S6K , mTOR, and Akt phosphorylation, effects that were completely prevented by the PI3K inhibitor, LY294002 ... Insulin induced phosphorylation of p70S6K and mTOR was prevented by the mTOR inhibitor, rapamycin
Zhou et al., Carcinogenesis 2007 (Neovascularization, Pathologic...) : In this study, we demonstrated that insulin induced H2O2 production and p70S6K1 activation in PC-3 prostate cancer cells ... The inhibition of H2O2 production by catalase abolished insulin induced p70S6K1 activation ... These results suggest that insulin induced HIF-1alpha and VEGF expression through H2O2 production and p70S6K1 activation in prostate cancer cells
Law et al., J Biol Chem 2000 : Interestingly, low salicylate concentrations ( < /=250 microm ) inhibit p70 ( s6k ) activation by phorbol myristate acetate, while higher salicylate concentrations ( > /=5 mm ) are required to block p70 ( s6k ) activation by epidermal growth factor + insulin-like growth factor-1
Grzelkowska-Kowalczyk et al., Pol J Vet Sci 2006 : Exposure to TNF-alpha but not IL-1beta impairs insulin dependent phosphorylation of protein kinase B and p70S6k in mouse C2C12 myogenic cells ... In cells differentiated in the presence of TNF-alpha an approximately 2-fold decrease of insulin mediated p70S6k phosphorylation was noted ... In conclusion : i ) high concentrations of TNF-alpha, but not IL-beta, present in the extracellular environment during myoblast differentiation prevent the stimulatory action of insulin on glucose uptake and protein synthesis ; ii ) insulin resistance induced by TNF-alpha in C2C12 myogenic cells could be associated with the decreased insulin mediated phosphorylation of PKB and p70s6k , but not with the basal phosphorylation of p42MAPK
Kim et al., Biochem Biophys Res Commun 1997 : There was also a rapid doubling of nuclear p70S6K activity in immunocomplex assays followed by a return to baseline by 30 min. Simultaneously, insulin stimulated cytoplasmic p70S6K by almost 10-fold at 10 min, and activity remained high up to 30 min. Tetradecanoylphorbol acetate ( TPA ) and fetal calf serum also stimulated nuclear p70S6K as judged by gel mobility shift ... Thus, nuclear p70S6K is regulated by insulin , serum and TPA
Wang et al., J Biol Chem 2007 : In response to insulin and nutrients, mTORC1, consisting of mTOR, raptor ( regulatory associated protein of mTOR ), and mLST8, is activated and phosphorylates eukaryotic initiation factor 4E-binding protein ( 4EBP ) and p70 S6 kinase to promote protein synthesis and cell size
Sherwood et al., Am J Physiol 1999 : Differential regulation of MAP kinase, p70 ( S6K ), and Akt by contraction and insulin in rat skeletal muscle ... However, insulin , but not contraction, increased p70 ( S6K ) and Akt activities in the muscle
Brennesvik et al., Cell Signal 2005 : In conclusion, adrenaline potentiates insulin stimulated activation of PKB and p70 ( S6K ) via cAMP and Epac in skeletal muscle
Zhou et al., J Biol Chem 2010 : Furthermore, adenovirus mediated expression of PHLDB1 in adipocytes enhanced insulin stimulated Akt and p70 S6 kinase phosphorylation, as well as GLUT4 translocation
Guillen et al., Endocrinology 2006 : In beta-IRLoxP beta-cells, p44/p42 MAPK and phosphatidylinositol 3 kinase pathways, mammalian target of rapamycin (mTOR), and p70S ( 6 ) K phosphorylation and beta-cell proliferation were stimulated in response to insulin
Shepherd et al., Biochem J 1995 : We have investigated the involvement of phosphoinositide (PI) 3-kinase and p70 ribosomal protein-S6 kinase (p70s6k) in mediating insulin stimulation of glycogen synthesis in 3T3-L1 adipocytes using specific inhibitors ... Rapamycin, which blocks insulin stimulated activation of p70s6k , decreased insulin activation of glycogen synthase in a dose dependent manner ( IC50 approximately 0.8 ng/ml ), with a maximum approx. 75 % inhibition of insulin 's stimulatory effect
Sale et al., FEBS Lett 1999 : Role of ERK1/ERK2 and p70S6K pathway in insulin signalling of protein synthesis
Oleksiewicz et al., J Appl Toxicol 2011 : By western blotting with phospho-specific antibodies, insulin induced phosphorylation of IRS-1, Akt, p70S6K , S6 ribosomal protein, 4E-BP1, FoxO3a, FoxO1, p44/42 MAPK and the EGFR
Grzelkowska-Kowalczyk et al., Pol J Vet Sci 2005 (Diabetes Mellitus, Experimental...) : Neither protein level nor insulin stimulated p70 ( S6k ) activation were modified by STZ-diabetes
Long et al., Am J Physiol Endocrinol Metab 2001 : DEX did not affect basal PHAS-I or p70 ( S6k ) phosphorylation but blocked insulin stimulated phosphorylation of PHAS-I- and amino acid stimulated phosphorylation of both PHAS-I and p70 ( S6k ) ( P < 0.01, for each )
Lynch et al., Am J Physiol Endocrinol Metab 2001 : Here it is shown that phenanthroline, a zinc and heavy metal chelator, inhibited both amino acid- and insulin stimulated phosphorylation of p70 ( S6k ) ... Both amino acid and insulin activations of p70 ( S6k ) involve a rapamycin-sensitive step that involves the mammalian target of rapamycin ( mTOR, also known as FRAP and RAFT )
Susa et al., J Biol Chem 1992 : Inhibition or down-regulation of protein kinase C attenuates late phase p70s6k activation induced by epidermal growth factor but not by platelet derived growth factor or insulin
Scott et al., J Biol Chem 1998 : These findings support the interpretation that increasing cAMP attenuates the effects of insulin on PHAS-I, p70 ( S6K ), and other downstream targets of the mTOR signaling pathway by inhibiting the phosphorylation and activation of mTOR
Valverde et al., FEBS Lett 2000 : However, activation of Akt/PKB or p70 S6 kinase ( p70 ( s6k ) ) by insulin remained unaltered
Fox et al., Invest Ophthalmol Vis Sci 2012 (Diabetes Mellitus, Experimental...) : The biochemical and biophysical mechanisms underlying the actions of PA on insulin activated mTOR/p70 S6Kinase signaling were determined using R28 retinal neuronal cells
Pullen et al., Science 1998 : More importantly, PDK1 activated p70s6k in vitro and in vivo, whereas the catalytically inactive PDK1 blocked insulin induced activation of p70s6k
Galbaugh et al., BMC cell biology 2006 : The activation of p70S6K by insulin or EGF resulted in the phosphorylation of ribosomal protein S6 (RPS6), elongation initiation factor 4E ( elF4E ) and 4E binding protein1 (4E-BP1)
Lee et al., J Biol Chem 2005 : We also show that insulin stimulated the phosphorylation of 4E-binding protein 1 (4E-BP1) and p70S6 kinase (p70S6K) in a mTOR dependent manner
Kim et al., J Pharmacol Exp Ther 2006 : These results show that PI3K/Akt/p70S6K signaling is active in the insulin mediated up-regulation of the antioxidant defense system and that low insulin levels, as encountered in diabetes, potentially increase the susceptibility of hepatocytes to xenobiotic mediated and/or oxidative stress mediated damage
Conejo et al., J Cell Physiol 2001 (MAP Kinase Signaling System) : Insulin stimulated IRS-1 association with p85alpha leading to the activation of PI3-kinase, and, subsequently AKT and p70S6-kinases
Chang et al., J Biol Chem 1995 (Insulin Resistance...) : In normal mice, insulin stimulated MAP kinase ( 6-fold ), p90rsk ( RSK2, 5-fold ), p70S6k ( 10-fold ), and glycogen synthase ( 30-50 % increase in fractional velocity )
Duchêne et al., Domest Anim Endocrinol 2008 : Similarly, refeeding and insulin increased the phosphorylation of p70S6K on key residues ( i.e. T389, T229 and T421/S424 ) and the phosphorylation of a p70S6K downstream target, the ribosomal protein S6 ( by 3-10-fold, P < 0.05 )
Mukherjee et al., Arch Biochem Biophys 1999 : However, only ethanol, but not bombesin, enhanced the stimulatory effect of insulin on p70 S6 kinase activity ; this effect of ethanol was zinc dependent