Overview
This super-track combines related tracks of DNaseI
sensitivity data from Duke University.
These tracks contain DNaseI analysis of CD4+ T-cells, using DNase-sequencing
and DNase-chip methods.
CD4+ T-cells, also known as helper or inducer T cells,
are involved in generating an immune response.
CD4+ T-cells are also one of the primary targets of the HIV virus.
DNaseI has long been used to map general chromatin accessibility and
the DNaseI "hyperaccessibility" or "hypersensitivity"
that is a universal feature of active cis-regulatory sequences.
The use of this method has led to the discovery of functional
regulatory elements that
include enhancers, insulators, promotors, locus control regions and novel
elements. DNaseI hypersensitivity signifies chromatin accessibility following
binding of trans-acting factors in place of a canonical nucleosome,
and is a universal feature of active cis-regulatory sequences in vivo.
Credits
These annotations were created by Alan Boyle, Terry Furey, and Greg
Crawford at Duke University's Institute for Genome Sciences & Policy (IGSP).
References
Boyle AP, Davis S, Shulha HP, Meltzer P, Margulies EH, Weng Z, Furey TS,
Crawford GE.
High-resolution mapping and characterization of open chromatin
across the genome. Cell. 2008 Jan 25;132(2):311-22.
Crawford GE, Davis S, Scacheri PC, Renaud G, Halawi MJ, Erdos MR,
Green R, Meltzer PS, Wolfsberg TG, Collins FS. DNase-chip: a high-resolution method to identify DNase I
hypersensitive sites using tiled microarrays. Nat
Methods. 2006 Jul;3(7):503-9.
Crawford GE, Holt IE, Whittle J, Webb BD, Tai D, Davis S, Margulies
EH, Chen Y, Bernat JA, Ginsburg D, Zhou D, Luo S, Vasicek TJ, Daly MJ,
Wolfsberg TG, Collins FS. Genome-wide mapping of DNase hypersensitive sites using
massively parallel signature sequencing (MPSS). Genome
Res. 2006 Jan;16(1):123-31.
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