Description
Serial analysis of gene expression (SAGE)
is a quantitative measurement of gene expression. Data are presented for every
cluster contained in the browser window and the selected cluster name is
highlighted in the table. All data are from the repository at the
SageMap project
built on UniGene version Hs 171. Click on a UniGene cluster name on the track
details page to display SageMap's page for that cluster. Please note that data
are not available for every cluster. There is no data available for clusters
that lie entirely within the bounds of larger clusters.
Methods
SAGE counts are produced by sequencing small "tags" of DNA believed
to be associated with a gene. These tags were generated by attaching
poly-A RNA to oligo-dT beads. After synthesis of double-stranded cDNA,
transcripts were cleaved by an anchoring enzyme (usually NlaIII). Then, small
tags were produced by ligation with a linker containing a type IIS restriction
enzyme site and cleavage with the tagging enzyme (usually BsmFI). The
tags were concatenated together and sequenced. The frequency of each
tag was counted and used to infer expression level of transcripts that could
be matched to that tag.
Credits
All SAGE data presented here were mapped to UniGene transcripts by the
SageMap project at NCBI.
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